| 凡纳滨对虾肠道微生物宏基因组Solexa 测序及其初步分析 |
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| 引用本文： | 严雪平,袁剑波,刘 斌.凡纳滨对虾肠道微生物宏基因组Solexa 测序及其初步分析[J].海洋科学,2012,36(6):9-14. |
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| 作者姓名： | 严雪平 袁剑波 刘 斌 |
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| 作者单位： | 1. 中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071
2. 中国科学院研究生院,北京100049 |
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| 基金项目： | 国家高技术研究发展计划(2007AA09Z444) |
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| 摘 要： | CTAB-酚/氯仿法提取新鲜凡纳滨对虾(Litopenaeus vannamei)肠道微生物宏基因组 DNA.结果表明: CTAB-酚/氯仿法提取总 DNA 的浓度达到92.5 ng/μL,半定量 PCR 法检测微生物基因组相对含量为69.9%.凡纳滨对虾肠道微生物宏基因组用于 Solexa 测序,生物信息学分析结果显示宏基因数据中64.1%的数据属于未知序列,35.5%属于真核生物,而已知的微生物和病毒序列所占比例仅有0.4%
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| 关 键 词： | 肠道微生物宏基因组 CTAB Solexa 测序 |
| 收稿时间： | 2011/4/15 0:00:00 |
| 修稿时间： | 2011/5/16 0:00:00 |
Solexa sequencing and analysis of the intestinal microorganisms metagenome in Litopenaeus vannamei |
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| YAN Xue-ping,YUAN Jian-bo,LIU Bin.Solexa sequencing and analysis of the intestinal microorganisms metagenome in Litopenaeus vannamei[J].Marine Sciences,2012,36(6):9-14. |
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| Authors: | YAN Xue-ping YUAN Jian-bo LIU Bin |
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| Institution: | 1(1.Key Laboratory of Experimential Marine Biology,Institute of Oceanology,Chinese Academy of Science,Qingdao 266071,China;2.Graduate University of Chinese Academy of Science,Beijing 100049,China) |
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| Abstract: | The intestinal microorganisms metagenome of Litopenaeus vannamei was extracted by CTAB-phenol/ chloroform DNA extraction protocol. The relative concentration of bacterial genome in metagenome was analyzed by semi-quantitative PCR. The results showed that the total concentration of metagenome was 92.5 mg/L and relative microbial metagenome ratio was 69.9%. Solexa sequencing showed that up to 64.1% of contigs in metagenome had no assignment or no hits in database of NCBI, 35.5% of which were assigned to eukaryon, and only 0.4% sequences belonged to bacteria and virus. |
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| Keywords: | intestinal microfloar metagenome CTAB solexa sequencing |
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