来源于印度洋深海区的烟曲霉次生代谢产物的研究

为了研究烟曲霉次生代谢产物,我们采用硅胶柱层析、sephadex LH-20凝胶柱层析、半制备液相色谱等分离方法对来源于印度洋深海区的烟曲霉(Aspergillus fumigatus)的乙醇提取液进行系统的次生代谢产物分离,根据所分离得到的化合物的理化性质和波谱数据确定化合物的结构。共分离鉴定了15个化合物,其中11个二酮哌嗪类生物碱(diketopiperazine),4个喹唑啉类衍生物(fumiquinazoline),分别为:fumitremorgin B(1),13-oxofumitremorgin B(2),fumitremorgin C(3),12-hydroxyfumitremorgin(13),3-hydroxyfumiquinazoline A(14)和fumiquinazoline E(15)。分离得到的15个化合物,丰富了烟曲霉次生代谢产物的类型,为烟曲霉的开发利用提供了化学研究基础。

Study of the secondary metabolites of Aspergillus fumigatus from the deepsea zone of the Indian Ocean

This study was conducted to analyze the chemical constituents of the secondary metabolites of Aspergillus fumigatus for which the chemical constituents of the ethanol extract of A. fumigatus from the deep sea zone of the Indian Ocean were separated systematically using silica gel column chromatography, Sephadex LH-20 column chromatography, and RP-HPLC. Physicochemical properties and spectral data were used to determine the structures of the isolated compounds. A total of 15 compounds were isolated that were identified as fumitremorgin B (1), 13-oxofumitremorgin B (2), fumitremorgin C (3), 12-hydroxyfumitremorgin C (4), cyclotyprostatins B (5), cyclotyprostatins C (6), cyclotyprostatins E (7), verruculogen (8), spirotryprostatin C (9), spirotryprostatin D (10), spirotryprostatin F (11), fumiquinazoline C (12), fumiquinazoline D (13), 3-hydroxyfumiquinazoline A (14), and fumiquinazoline E (15). These 15 compounds isolated from A. fumigatus enriched the type of the secondary metabolites of the fungus and provided a basis for exploring its development and conducting chemical research.