| 烟台海域中度嗜盐菌分离、鉴定和系统进化研究 |
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| 引用本文： | 冯二梅,宿红艳,王磊.烟台海域中度嗜盐菌分离、鉴定和系统进化研究[J].海洋通报,2010,29(1). |
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| 作者姓名： | 冯二梅 宿红艳 王磊 |
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| 作者单位： | 鲁东大学生命科学学院,山东,烟台,264025 |
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| 基金项目： | 国家自然科学基金项目(30740017);;鲁东大学学科建设项目 |
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| 摘 要： | 为研究山东烟台海域中度嗜盐菌的分布情况及种群特征,从烟台近海盐场采集的土样和水样中利用高氏一号培养基分离得到22株中度嗜盐菌。通过菌落形态观察、生理生化特征分析比较,选取其中的9株进行16SrDNA序列分析和系统进化分析,结果表明这些中度嗜盐菌分别属于7个属:芽孢杆菌属(Bacillus)、枝芽孢杆菌属(Virgibacillus)、薄壁芽孢杆菌属(Gracilibacillus)、刘氏菌属(Liuella)、产微球茎菌属(Microbulbifer)、拟诺卡氏菌属(Nocardiopsis)和交替单胞菌属(Alteromonas),其中菌株YTM-8、YTM-12和YTM-13可能是潜在的新种。以上研究结果表明烟台海域中存在有较为丰富的嗜盐微生物多样性,并且潜藏着新类型的嗜盐菌资源。
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| 关 键 词： | 烟台海域 嗜盐菌 16SrDNA 生物多样性 系统进化分析 |
Isolation, identification and phylogenetic analysis of moderately halophilic bacterium isolated from Yantai Sea area |
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| FENG Er-mei,SU Hong-yan,WANG Lei.Isolation, identification and phylogenetic analysis of moderately halophilic bacterium isolated from Yantai Sea area[J].Marine Science Bulletin,2010,29(1). |
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| Authors: | FENG Er-mei SU Hong-yan WANG Lei |
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| Institution: | College of Life Sciences;Ludong University;Yantai 264025;China |
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| Abstract: | To study the distribution and characteristics of moderately halophilic bacteria,the samples were collected from salt field near Yantai sea area in Shandong province.22 ocean moderately halophilic bacteria strains were isolated on Gause I agar medium.Based on the morphological,physiological and biochemical analysis,the 9 strains were chosen for 16 S rDNA amplification and phylogenetic analysis.Phylogenetic analysis based on 16 S rDNA gene sequences indicated that these moderately halophilic bacteria strains ... |
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| Keywords: | Yantai sea area halophilic bacterium 16S rDNA biodiversity phylogenetic analysis |
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