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| 长牡蛎基因组微卫星引物的开发和特性描述 | |
| 引用本文： | 韩斐斐,亓海刚,李莉,张国范,闫喜武,李慧娟,杨霏,王琳楠.长牡蛎基因组微卫星引物的开发和特性描述[J].海洋通报,2011,30(5):566-571. |
| 作者姓名： | 韩斐斐 亓海刚 李莉 张国范 闫喜武 李慧娟 杨霏 王琳楠 |
| 作者单位： | 1. 大连海洋大学,辽宁大连116023;中国科学院海洋研究所,山东青岛266071 2. 中国科学院海洋研究所,山东青岛,266071 3. 大连海洋大学,辽宁大连,116023 |
| 基金项目： | 国家重点基础研究发展计划（973计划项目，2010CB126402）；国家自然基金（40730845）；国家公益性行业(农业)科研专项（3-53）；中国博士后基金（20090461270） |
| 摘 要： | 长牡蛎于20世纪80年代从日本引进中国.在我国,长牡蛎已经成为重要的贝类养殖产业之一.本实验从全基因组上筛查微卫星序列,在微卫星筛查的范围、数目和类型上是传统的富集文库法开发微卫星所无法比拟的.利用基因组微卫星序列总共设计了104对引物,54对引物能扩增出目的片段,其中有34对引物显示多态性扩增,占32.7％,20对引... |
| 关 键 词： | 长牡蛎 微卫星 标记开发 基因组 |
| 收稿时间： | 2011/3/11 0:00:00 |
| 修稿时间： | 2011/5/11 0:00:00 |
Development and characterization of SSR in Genome for the Pacific Oyster Crassostrea gigas |
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| HAN Fei-fei,QI Hai-gang,LI Li,ZHANG Guo-fan,YAN Xi-wu,LI Hui-juan,YANG Fei and WANG Lin-nan.Development and characterization of SSR in Genome for the Pacific Oyster Crassostrea gigas[J].Marine Science Bulletin,2011,30(5):566-571. | |
| Authors: | HAN Fei-fei QI Hai-gang LI Li ZHANG Guo-fan YAN Xi-wu LI Hui-juan YANG Fei and WANG Lin-nan |
| Institution: | 1. Dalian Ocean University, Dalian 116023, China; 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Dalian Ocean University, Dalian 116023, China;1. Dalian Ocean University, Dalian 116023, China; 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Dalian Ocean University, Dalian 116023, China;Dalian Ocean University, Dalian 116023, China |
| Abstract: | The Pacific Oyster(Crassostrea gigas) was introduced into China from Japan in 1980s.Since then,the Pacific Oyster culture has been one of the important mollusks in China.Microsatellite(Simple Sequence Repeats,SSRs) sequences were screened from the whole genome,which has advantage over the traditional microsatellite-screening method including the scope,number and type of microsatellites.Primers were designed for one hundred and four SSR loci derived from genome microsatellite sequences.Fifty-four primer pair... |
| Keywords: | Crassostrea gigas Microsatellite Marker development Genome |
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