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| PCR法制备地高辛标记探针斑点杂交 检测白斑综合症病毒(WSSV) | |
| 引用本文： | 雷质文,史成银,黄倢,杨冰,俞开康,战文斌.PCR法制备地高辛标记探针斑点杂交 检测白斑综合症病毒(WSSV)[J].中国海洋大学学报(自然科学版),2001,31(2):201-204. |
| 作者姓名： | 雷质文 史成银 黄倢 杨冰 俞开康 战文斌 |
| 作者单位： | 1. 青岛海洋大学水产学院;中国水产科学研究院黄海水产研究所 2. 中国水产科学研究院黄海水产研究所, 3. 青岛海洋大学水产学院, |
| 基金项目： | 国家“九五”科技攻关项目（96-005-01-02）资助 |
| 摘 要： | 用PCR法成功制备了DIG标记探针，探针长度为547bp，探针的产量为21.6ng/μL。此探针与随机引物合成探针检测样品灵敏度相近。用此探针核酸斑点杂交法检测了54尾中国对虾。结果表明此探针在对白斑综合症病毒的检测、对虾暴发性流行病的诊断等方面具有很高的应用价值。 |
| 关 键 词： | 多聚酶链式反应(PCR) 核酸探针 白斑综合症病毒(WSSV) 中国对虾 |
| 文章编号： | 1001-1862(2001)02-201- 04 |
| 修稿时间： | 2000年7月6日 |
Development of Digoxigenin Labeled Probe for Detection of White Spot Syndrome by Dot-blot Hybridization |
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| Lei Zhiwen , Shi Chengyin Huang Jie Yang Bing Yu Kaikang Zhan Wenbin.Development of Digoxigenin Labeled Probe for Detection of White Spot Syndrome by Dot-blot Hybridization[J].Periodical of Ocean University of China,2001,31(2):201-204. | |
| Authors: | Lei Zhiwen Shi Chengyin Huang Jie Yang Bing Yu Kaikang Zhan Wenbin |
| Institution: | Lei Zhiwen 1,2 Shi Chengyin2 Huang Jie2 Yang Bing2 Yu Kaikang1 Zhan Wenbin1 |
| Abstract: | A digoxigenin labeled DNA probe was produced by PCR. The s ize ofprobe was 547bp, and its production was 21.6ng/μL. The results of dot blot hybridization by the probe developed by PCR were the same as those by th e probe of kit produced by random primer, their sensitivity were similar. Using the probe produced by PCR to detection 54 P. chinensis by dot blot hybri dization showed that the probe produced by PCR was h-a valuable in detecting WS SV and diagnosing explosive epidemic disease. |
| Keywords: | PCR DNA probe WSSV Penaeas chineneis |
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