| 高效毛细管电泳法测定海参多糖的单糖组成 |
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| 引用本文： | 杨洁,姜廷福,王远红,吕志华.高效毛细管电泳法测定海参多糖的单糖组成[J].中国海洋大学学报(自然科学版),2011(Z1):344-348. |
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| 作者姓名： | 杨洁 姜廷福 王远红 吕志华 |
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| 作者单位： | 中国海洋大学医药学院; |
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| 基金项目： | 国家海洋局海洋公益性项目(201005024)资助 |
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| 摘 要： | 目的:采用高效毛细管电泳法测定3种不同海参多糖的单糖组成。方法:海参多糖水解后,经PMP衍生化,高效毛细管电泳分析。电泳条件:缓冲液为60 mmol.L-1硼砂溶液(pH=9.0);柱温25℃;运行电压25 kV;检测波长245 nm;压力进样50 mbar,进样时间5 s。结果:3种不同海参多糖中均含GalN、GlcN、GalNAC、Glc、Man、Fuc、Gal、GlcUA,梅花参多糖中各单糖的摩尔比为1.00∶1.02∶1.63∶1.19∶1.02∶10.26∶0.82∶0.38,四方刺参多糖中各单糖的摩尔比为1.00∶0.41∶0.43∶0.56∶0.29∶5.47∶0.43∶0.25,北极参多糖中各单糖的摩尔比为1.00∶0.26∶0.22∶0.25∶0.06∶1.36∶0.27∶0.16。结论:该方法具有简单、快速、分离效率高等特点,能同时测定海参多糖中氨基糖、中性糖、糖醛酸的组成。
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| 关 键 词： | 海参多糖 单糖组成 高效毛细管电泳 |
Determination of Monosaccharide Composition in Sea Cucumber Polysaccharides by High Performance Capillary Electrophoresis |
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| YANG Jie,JIANG Ting-Fu,WANG Yuan-Hong,LV Zhi-Hua.Determination of Monosaccharide Composition in Sea Cucumber Polysaccharides by High Performance Capillary Electrophoresis[J].Periodical of Ocean University of China,2011(Z1):344-348. |
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| Authors: | YANG Jie JIANG Ting-Fu WANG Yuan-Hong LV Zhi-Hua |
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| Institution: | YANG Jie,JIANG Ting-Fu,WANG Yuan-Hong,LV Zhi-Hua(School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | A HPCE method had been established for analysis of the monosaccharide compositions of polysaccharides from three kinds of sea cucunmber.These monosaccharides were derived with 1-pheny-3-methyl-5-pyrazolone(PMP) after the polysaccharides are degraded by trifluoroacetic acid(TFA).The derived monosaccharides were detected by a diodearray detecter at 245 nm.The eight monosacchrides Galactosamine(GalN),Glucosamine(GlcN),N-acetylgalatosamine(GalNAc),Glucose(Glc),Mannose(Man),Fucose(Fuc),Galactose(Gal),Glucuronic ... |
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| Keywords: | sea cucumber monosaccharide composition high performance capillary electrophoresis |
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