| 两种海洋微藻硝酸还原酶活性测定方法的比较研究 |
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| 引用本文： | 唐洪杰,王修林,祝陈坚,李雁宾,张传松.两种海洋微藻硝酸还原酶活性测定方法的比较研究[J].中国海洋大学学报(自然科学版),2006,36(6):981-986. |
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| 作者姓名： | 唐洪杰 王修林 祝陈坚 李雁宾 张传松 |
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| 作者单位： | 1. 中国海洋大学,海洋化学理论与工程技术教育部重点实验室,山东,青岛,266100;中国海洋大学,化学化工学院,山东,青岛,266100
2. 中国海洋大学,海洋化学理论与工程技术教育部重点实验室,山东,青岛,266100;中国海洋大学,化学化工学院,山东,青岛,266100;中国海洋大学,环境科学与工程学院,山东,青岛,266100 |
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| 基金项目： | 国家自然科学基金;国家自然科学基金 |
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| 摘 要： | 对6种常见海洋微藻的硝酸还原酶活性测定方法进行了初步研究。确立了离体法和活体法的提取(振荡)时间及酶促反应时间,分别为:5 min,30 min(离体法)和6 min,10 min(活体法),并对2种方法进行了对比。结果表明:在本文条件下,离体法较活体法更适于进行塔玛亚历山大藻(Alexandrium tamarense)、强壮前沟藻(Amphidinium carterae)、中肋骨条藻(Skeletonema costatum)、新月菱形藻(Nitzchia closterium)和旋链角毛藻(Chaetoceros curvisetus)的硝酸还原酶活性的测定;活体法更适于东海原甲藻(Prorocentrum donghainase)硝酸还原酶活性的测定。
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| 关 键 词： | 海洋微藻 硝酸还原酶 硝酸还原酶活性 离体法 活体法 |
| 文章编号： | 1672-5174(2006)06-981-06 |
| 收稿时间： | 2006-04-11 |
| 修稿时间： | 2006-09-16 |
Comparison Studies on Two Testing Methods of Nitrate Reductase Activity in Marine Microalgae |
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| TANG Hong-Jie,WANG Xiu-Lin,ZHU Chen-Jian,LI Yan-Bin,ZHANG Chuan-Song.Comparison Studies on Two Testing Methods of Nitrate Reductase Activity in Marine Microalgae[J].Periodical of Ocean University of China,2006,36(6):981-986. |
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| Authors: | TANG Hong-Jie WANG Xiu-Lin ZHU Chen-Jian LI Yan-Bin ZHANG Chuan-Song |
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| Institution: | Ocean University of China 1. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education; 2. College of Chemistry and Chemical Engineering; 3. College of Environment Science and Engineering, Qingdao 266100, China |
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| Abstract: | The NRA of 6 species of marine microalgae was investigated by in vitro and in vivo methods.The optimum homogenized time and enzymecatalyzed time,i.e.5 min and 30 min respectively for the in vitro method versus the optimum agitated time and enzyme-catalyzed time,i.e.6 min and 10 min respectively for the in vivo method were determined.A comparison of the two testing methods was studied;the results suggested that the in vitro method was more suitable for measuring NRA in Alexandrium tamarense,Amphidinium carterae,Skeletonema costatum,Nitzchia closterium and Chaetoceros curvisetus,whereas the in vivo method was more suitable for assaying NRA in Prorocentrum donghainase under the experimental conditions in this study. |
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| Keywords: | marine microalgae nitrate reductase nitrate reductase activity in vitro method in vivo method |
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