| 以rDNA为同源重组位点酵母表达鲑鱼降钙素基因多拷贝整合载体的构建 |
| |
| 引用本文： | 姜勇,张学成,孙平楠,陈云松.以rDNA为同源重组位点酵母表达鲑鱼降钙素基因多拷贝整合载体的构建[J].中国海洋大学学报(自然科学版),2009,39(3). |
| |
| 作者姓名： | 姜勇 张学成 孙平楠 陈云松 |
| |
| 作者单位： | 1. 中国海洋大学海洋生命学院,山东,青岛,266003
2. 汕头大学医学院,广东,汕头,515071 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 根据酵母整合质粒的设计要求,PCR扩增2.2 kb rDNA片段,导入酿酒酵母(Saccharomyces cerevisiae)整合载体pYD1中,设计并合成适合在酵母中表达的鲑鱼降钙素(Salmon Calcitonin),以此为目标基因,构建适合酿酒酵母多拷贝整合的表达载体p-r-sCT.通过载体p-r-sCT引入到酿酒酵母菌株EBY100中,得到酵母重组菌株yAGA2-r-sCT.流式细胞检测结果表明,目标基因鲑鱼降钙素在重组菌株的yAGA2-r-sCT中得到了表达,实现了外源基因在酿酒酵母菌株中的稳定表达.
|
| 关 键 词： | 鲑鱼降钙素 酿酒酵母 多拷贝整合 流式细胞技术 |
Construction of a Ribosomal DNA Multi-Copy Integration Vector for Saccharomyces cerevisiae Expressing Salmon Calcitonin |
| |
| JIANG Yong,ZHANG Xue-Cheng,SUN Ping-Nan,CHEN Yun-Song.Construction of a Ribosomal DNA Multi-Copy Integration Vector for Saccharomyces cerevisiae Expressing Salmon Calcitonin[J].Periodical of Ocean University of China,2009,39(3). |
| |
| Authors: | JIANG Yong ZHANG Xue-Cheng SUN Ping-Nan CHEN Yun-Song |
| |
| Institution: | 1.College of Marine Life Sciences;Ocean University of China;Qindao 266003;China;2.Medical College of Shantou University;Shantou 515071;China |
| |
| Abstract: | We cloned Salmon Calcitonin(sCT) gene and a 2.2 kb rDNA fragment from Saccharomyces cerevisiae into pYD1 vector,created a new multi-copy intergration plasmid p-r-sCT.This plasmid was gene transformed into Saccharomyces cerevisiae EBY100 and obtained a new strain Saccharomyces cerevisiae yAGA2-r-sCT.Fluorescent pictures indicated that Salmon Calcitonin was expressed in yAGA2-r-sCT and the quantity of expression was detected by flow cytometry,which means that the sCT gene was integrated into the yast chromoso... |
| |
| Keywords: | rDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
