低盐胁迫下松江鲈aquaporin基因的表达变化规律

为了探究aquaporin基因家族成员在松江鲈(Trachidermus fasciatus)应对低盐胁迫过程中的调节作用，本研究选取7个Aquaporin基因（aqp1、aqp4、aqp7、aqp8、aqp10、aqp11和aqp12）为目标基因，利用实时荧光定量PCR技术检测了鳃、肠、肾和肝组织中7个基因在两种低盐胁迫处理下不同时间点(0 h、12 h、24 h和48 h)的表达水平。在两种低盐胁迫处理下，aqp4在松江鲈鳃、肝和肠组织中无表达，aqp7在鳃、肝组织中无表达。此外，其他aqp基因在四个组织中的表达量变化趋势具有显著差异。除肾组织中aqp12和肠组织中的aqp7、aqp8和aqp10之外，特定组织中aquaporin基因在两种低盐胁迫下呈现相似的表达量变化趋势。本研究通过比较分析了aquaporin基因在松江鲈应对不同低盐胁迫时表达变化规律的异同，相关结果为探讨这一家族成员在鱼类应激调节过程中的作用及洄游性鱼类适应盐度变化的分子调控机制提供了理论依据。

Effects of osmotic stress on the expression profiling of aquaporin genes in the roughskin sculpin (Trachidermus fasciatus)

Aquaporins (AQPs) are a family of integral membrane proteins that have been shown to be important for osmoregulation in many vertebrates. To identify potential stress resistance-related aqp genes in salinity adaptation of the roughskin sculpin Trachidermus fasciatus, we investigated the time-course expression dynamics of seven aquaporin genes (aqp1, 4, 7, 8, 10, 11 and 12) in three osmoregulatory tissues (kidney, gill and intestine) and one metabolic tissue (liver). The fish were subjected to two different acute osmotic treatments (seawater-to-freshwater transfer respectively achieved in 1 h and 24 h, namely, E-acute and acute group). The expression profiling of the seven aqp genes were performed using quantitative real-time PCR (qRT-PCR). At the time of all sampling time points (0 h, 12 h, 24 h and 48 h), no expression of aqp4 was found in the gill, liver and intestine; no expression of aqp7 was found in the gill and liver. Significant differences of aqp expression were determined in the four target tissues, and the mRNA levels were largely variable among gene members and tissues. Similar patterns of the time-course expression were detected in most of the aqp genes in T. fasciatus between the two acute groups, except that only one gene (aqp12) in the kidney and three genes (aqp7, aqp8 and aqp10) in the intestine revealed different expression patterns. These results suggest that the expression response of aqp genes was similar under osmotic changes with different rates.