南麂岛海洋沉积物中抗大黄鱼(Pseudosciaena crocea)致病弧菌的放线菌分离和筛选研究

采用平板涂布法从南麂岛近海海洋沉积物样品中分得86株放线菌,用琼脂块法和杯碟法结合筛选出对副溶血弧菌(Vibrio parahaemolyticus)、哈维氏弧菌(V.harveyi)两种大黄鱼病原性弧菌具有不同拮抗作用的海洋放线菌5株,其中编号NJR0956的菌株对两种致病菌的抑菌圈直径均25mm,表现出较强的抑制作用;进而测定了5株活性菌株的抗菌谱。结果表明,它们对指示菌中的副溶血弧菌、哈维氏弧菌、鳗弧菌(V.anguillarum)、溶藻弧菌(V.alginolyticus)四种病原弧菌均具有较好的拮抗作用;在对NJR0956的抑菌效果研究中发现,该菌株在牛肉膏蛋白胨液体培养基中不仅能够增殖,而且能够有效地抑制病原菌的生长。

ISOLATION OF MARINE ACTINOMYCETES STRAINS IN SEDIMENTS FROM NANJI ISLAND OFFSHORE AND THE ANTIMICROBIAL ACTIVITY OF THEIR METABOLITES AGAINST PATHOGENETIC VIBRIO FROM PSEUDOSCIAENA CROCEA

Eight-six actinomycetes strains were isolated from the marine sediments near Nanji island, China, by using the plate paint isolation method. Their antimicrobial activities against Pathogenetic Vibrio from Pseudosciaena crocea were tested by using both agar lump and cup-plate methods. Five actinomycetes strains of them could evidently inhibit the growth of two Pathogenetic Vibrio, V. parahaemolyticus and V. harveyi; and the antimicrobial ability of NJR0956 strain was relatively stronger, with the inhibitory circle of more than 25mm. In addition, the antibiotic ranges of five actinomycetes strains were investigated. Actinomycetes strain NJR0956 could evidently inhibit the growth of four kinds of Pathogen Vibrio. In this study, it was also discovered that NJR0956 strain could not only multiply rapidly but also inhibit the growth of Pathogen Vibrio effectively in meat broth.