| 4株米氏凯伦藻(Karenia mikimotoi)藻际异养细菌的分离鉴定 |
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| 引用本文： | 龚诗雁,屠燕萍,谢志浩.4株米氏凯伦藻(Karenia mikimotoi)藻际异养细菌的分离鉴定[J].海洋与湖沼,2014,45(5):1099-1104. |
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| 作者姓名： | 龚诗雁 屠燕萍 谢志浩 |
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| 作者单位： | 宁波大学海洋学院 宁波 315211; 宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;宁波大学海洋学院 宁波 315211; 宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;宁波大学海洋学院 宁波 315211; 宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211 |
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| 基金项目： | 浙江省科技厅项目, 2014C32074 号; 浙江省自然科学基金项目, LY12C03001 号; 宁波大学学科项目, XKL11D2100 号 |
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| 摘 要： | 采用分子生物学方法,对分离的米氏凯伦藻4株藻际异养细菌进行分子鉴定和功能分析。结果表明,经16S rRNA序列分析,4株藻际异养细菌分别隶属于Formosa、Erythrobacter、Shewanella和Marinobacter四个属,P1菌株与Formosa的16S rRNA核苷酸序列的同源性为95%,P2、P3和P4菌株与Erythrobacter、Shewanella和Marinobacter的同源性在99%以上。通过4株异养细菌及其同源性大的24株细菌构建进化树得出,28株细菌在系统发育树中主要分成4个分支,与同源性分析相符。
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| 关 键 词： | 米氏凯伦藻 藻际异养细菌 S rRNA 分子鉴定 |
| 收稿时间： | 2013/10/18 0:00:00 |
| 修稿时间： | 2014/2/25 0:00:00 |
MOLECULAR IDENTIFICATION OF FOUR STRAINS OF HETEROTROPHIC BACTERIA ISOLATED FROM KARENIA MIKIMOTOI |
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| GONG Shi-Yan,TU Yan-Ping and XIE Zhi-Hao.MOLECULAR IDENTIFICATION OF FOUR STRAINS OF HETEROTROPHIC BACTERIA ISOLATED FROM KARENIA MIKIMOTOI[J].Oceanologia Et Limnologia Sinica,2014,45(5):1099-1104. |
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| Authors: | GONG Shi-Yan TU Yan-Ping and XIE Zhi-Hao |
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| Institution: | School of Marine Science, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China;School of Marine Science, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China;School of Marine Science, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China |
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| Abstract: | Molecular identification and functional analysis on heterotrophic bacteria from Kareoia mikimotoi were carried out. Four cultivable strains, ie., P1 to P4 that selected from these isolates were identified and classified based on analysis of 16S rRNA sequence. The results show that they belong to four genera including, Erythrobacter, Shewanella, and Marinobacter. Similarity search in 16S rRNA sequence indicated that Strain P1 could be identified Formosa to genus level, and other three strains P2 to P4 to species level for sharing more than 99% sequence homology with some strains of Erythrobacter, Shewanella, and Marinobacter, respectively. Phylogenetic tree between the four strains and their 24 homologies could be divided into four branches, which is consistent with homology analysis. |
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| Keywords: | Karenia mikimotoi heterotrophic bacteria 16S rRNA molecular identification |
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