标记乳糖基白蛋白SPIO作为磁共振肝细胞受体显影剂的实验研究

目的是探讨标记乳糖基白蛋白的超顺磁性氧化铁粒子（ＬＡＣ－ＨＳＡ－ＳＰＩＯ）作为配体与肝细胞膜去唾液酸糖蛋白受体（ＡＳＧ）特异性结合可能性；评价ＬＡＣ－ＨＳＡ－ＳＰＩＯ增强磁共振受体成像对大鼠微小肝癌灶的显示能力。方法：将Ｆｅ３＋Ｆｅ２＋混合液与ＬＡＣ－ＨＳＡ在碱性条件下制备出ＬＡＯ－ＨＳＡ－ＳＰＩＯ；应用　二乙基亚硝酸胺建立大鼠肝癌模型；电镜及Ｐｅｒｌｓ（普鲁士蓝）染色，观察ＬＡＣ－ＨＳＡ－ＳＰＩＯ在肝组织的颁；并比较ＬＡＣ－ＨＳＡ－ＳＰＩＯ增强前后大鼠肝癌的显示情况。得到结果是（１）Ｐｅｒｌｓ染色证实有ＬＡＣ－ＨＳＡ－ＳＰＩＯ分布到肝细胞浆内；电镜下也显示肝细胞膜表面及胞浆内有电子致密ＳＰＩＯ粒子分布；（２）注射小剂量ＬＡＣ－ＨＳＡ－ＳＰＩＯ可观察到明显明显肝脏强化效果，大鼠肝癌检出率由平扫３６．８％（７／１９）提高到７８％（１５／１９，Ｐ＜００１）。结论：ＬＡＣ－ＨＳＡ－ＳＰＩＯ是一种新型的具有肝细胞ＡＳＧ受体靶向性显影剂，对提高肝脏微小瘤灶检出率有章要价值。

MR Receptor Imaging: Lactosaminated Human Serum Albumin Coated SPIO Targeted to Asialoglycoprotein Receptors

Objective: TO explore the possibility of lactosaminated human serum albumincoated superparamagnetic iron oxide particles (LAC-HSA-SPIO) as a legend forasialoglycoprotein receptor; and to assess the diagnostic roles of LAC- HSA-SPIO enhanced MRIin detection of micro-hepatocellular carcinoma (HCCs) in rat model. Method: LAC-HSA-SPIOwas sythesized by reaction the mixing of Fe~3+and Fe~2+ with LAC- HSA in alkaline condition. Therat model bearing HCCs in cirrhotic liver was established by oral administration ofdiethylnitrosamine. The distribution of LAC-HSA -SPIO in liver was observed by perls' prussianblue strain and electron microscopy. The rat models with HCCs was studieed with FSE sequencebefore and 30 minutes after injection of small dose of LAC-HSA-SPIO.Resuits: (1.)Histologically, stainable iron was noticed in hepatocytes, but to lesser degree, the iron stain wasalso shown in kupffer cells. By electron microscopy, LAC-HSA-SPIO was identified as electrondense particles on the surface of hepatocyte menbranes and in the cytoplasm of hepatocytes.(2.) a total of 19 foci were proven as HCCs pathologically. 78.9%(15/19)of the lesions wasdemonstrated on post contrast images, however, only 36.8%(7/19) of the lesions was found onprecontrast images (p<0.ol). Conclusion: Our data indicated that LAC- HSA -SPIO is specificityfor hepatic ASG receptors. LAC-HAS-SPIO appears to be a useful method for improvingdetection of micro HCCs,