| Vaccination in three different ways against vibriosis of Seriola dumerili caused by Vibrio hollisae |
| |
| 作者姓名： | 纪荣兴 邹文政 胡石柳 鄢庆枇 |
| |
| 作者单位： | Key Laboratory of Science and Technology for Aquaculture and Food Safety，Fisheries College，Jimei University，Xiamen 361021，China，Fisheries School of Jimei，Xiamen 361012，China |
| |
| 基金项目： | 国家自然科学基金，福建省科技攻关项目 |
| |
| 摘 要： | Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili (amberjack) suffering from vibriosis. Healthy S. dumerili were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1:40 at 7 d, and reached its peak of 1:320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of <1:10, 1:20, 1:160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities (P<0.05). Upon challenge with virulent strain, the relative percent survival (RPS) of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.
|
| 关 键 词： | 疫苗 弧菌 接种疫苗 水产养殖 |
Vaccination in three different ways against vibriosis of Seriola dumerili caused by Vibrio hollisae |
| |
| HU Shiliu.Vaccination in three different ways against vibriosis of Seriola dumerili caused by Vibrio hollisae[J].Chinese Journal of Oceanology and Limnology,2008,26(3). |
| |
| Authors: | HU Shiliu |
| |
| Institution: | [1]Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China [2]Fisheries School of Jimei, Xiamen 361012, China |
| |
| Abstract: | Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili (amberjack) suffering from vibriosis. Healthy S. dumerili were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1:40 at 7 d, and reached its peak of 1:320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of <1:10, 1:20, 1:160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities (P<0.05). Upon challenge with virulent strain, the relative percent survival (RPS) of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm. |
| |
| Keywords: | Seriola dumerili Vibrio hollisae vaccine vaccinate procedure protection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
