双棘黄姑鱼血清性类固醇激素的周年变化

采用放射免疫法(RIA)测定双棘黄姑鱼(Nibea diacanthus)血清性类固醇激素的周年变化。结果表明:雌鱼血清雌二醇(Estadiol-17β)浓度一年出现两次峰值,均在成熟产卵前一个月,即4月(338±59)pg/mL]和9月(296±48)pg/mL];血清睾酮(T)浓度在卵黄发生期急剧上升,并在卵巢成熟期达到最高值,分别是5月(2.506±0.262 ng/mL)和10月份(2.268±0.243 ng/mL),分别在血清雌二醇浓度两次峰值出现1个月之后,因此,雌鱼的血清雌二醇浓度和血清睾酮浓度均与性腺发育相关。雄鱼血清睾酮浓度的两次峰值均出现在性腺已发育成熟的5月份(2.307±0.792 ng/mL)和10月份(2.286±0.607 ng/mL),与性腺发育有明显的相关性;而血清雌二醇浓度全年维持在一个相对稳定的水平,与雄性性腺发育相关性不明显。

Seasonal Cycles of Serum Sex Steroid Levels of Nibea diacanthus

The seasonal cycles of sex steroid of Nibea diacanthus were determined by RIA in this article.The results showed that the females had two Estadiol-17β peak values in April(338±59pg/mL) and September(296±48pg/mL) respectively,just one month before the spawning season.The levels of serum Testosterone of females rose quickly during yolk formation period,and the levels of Testosteroneestosterone changed followed with that of Estadiol-17β, and reached its highest levels in the period of ovary maturation.The results showed that both Estadiol-17β and T had relationship with ovary development in females.The levels of serum Testosterone in males had two peak values in May(2.307±0.792ng/mL) and October(2.286±0.607ng/mL) respectively,just in the spawning seasons.It showed that the levels of serum Testosterone of males had relationship with spermary development.While the serum Estadiol-17β levels of males changed little during the seasonal cycles,it seemed the relationship between Estadiol-17β and spermary development was not obviously.