| EFFECT OF NITRATE AND PHOSPHATE ON ACCUMULATION OF β-CAROTENE ISOMERS IN DUNALIELLA SALINA |
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| 作者姓名： | 刘建国 吴超元 陈念洪 王玉君 俞立东 |
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| 作者单位： | Institute of Oceanology，Chinese Academy of Sciences，Qingdao 266071 |
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| 基金项目： | This project was supported by the National Natural Science Foundation of China,Grant No.38970587. |
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| 摘 要： | DunaIiella salina, a halotolerent unicellular green alga, can accurmulate a Iarge amount of β-caroteneunder environmental conditions. The isorners of β-carotene extIacted from D. salina culturedin medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-Talumins column. At least six isomers were found in different proportions depending on the culture me-dia's nitrate and/or phosphate concentrations. Nitrate and/or phosphae defidency was conducive tothe accumulation of totaI cis isomers but not of al1 trans isomer. lt is sUggeSted that 1 mmol/L KNO_3and 0.1 mmol/L KH_2, PO_4 are favourable for accumulation of total cis β-carotene.
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| 关 键 词： | Dunaliella salina β-carotene isomer cis and trans isomers accumulation nitrate and phosphate effect |
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