| 淋巴囊肿病毒中国分离株基因组序列和结构分析 |
| |
| 引用本文： | 闫秀英,吴灶和,简纪常,鲁义善,孙修勤.淋巴囊肿病毒中国分离株基因组序列和结构分析[J].广东海洋大学学报,2011,31(1):1-6. |
| |
| 作者姓名： | 闫秀英 吴灶和 简纪常 鲁义善 孙修勤 |
| |
| 作者单位： | 1. 广东省水产经济动物病原生物学及流行病学重点实验室,广东海洋大学,广东湛江524025
2. 海洋生态研究中心,国家海洋局第一海洋研究所,山东青岛266061 |
| |
| 基金项目： | 国家高技术研究发展计划项目 |
| |
| 摘 要： | LCDV-cn是分离自中国养殖牙鲆的淋巴囊肿病病原,给中国牙鲆养殖业带来了严重危害。通过PCR、克隆和测序,获得了LCDV-cn部分基因的序列,证明LCDV-cn与LCDV-C的基因组序列是一致的。比较基因组学分析发现,LCDV-cn基因组中有137个ORF是其独有的、与LCDV-1及其它虹彩病毒无同源区域。对LCDV-cn基因组进行生物信息学分析发现,在LCDV-cn独有的ORF中,有的编码与宿主同源的蛋白,有的编码与DNA复制相关的蛋白,某些ORF编码的蛋白含有跨膜区等。LCDV-cn的独有基因或许对其毒力和宿主范围有关。
|
| 关 键 词： | 淋巴囊肿病毒中国分离株LCDV-cn 比较基因组学 生物信息学 |
Analysis on Genome Sequence and Structure of Lymphocystis Disease Virus-Chinese Isolate |
| |
| YAN Xiu-ying,WU Zao-he,JIAN Ji-chang,LU Yi-shan,SUN Xiu-qin.Analysis on Genome Sequence and Structure of Lymphocystis Disease Virus-Chinese Isolate[J].Journal of Zhanjiang Ocean University,2011,31(1):1-6. |
| |
| Authors: | YAN Xiu-ying WU Zao-he JIAN Ji-chang LU Yi-shan SUN Xiu-qin |
| |
| Institution: | YAN Xiu-ying1,SUN Xiu-qin2,WU Zao-he1,LU Yi-shan,JIAN Ji-chang1(1.Guangdong Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals,Guangdong Ocean University,Zhanjiang 524025,China,2.First Institute of Oceanography SOA,Qingdao 266061,China) |
| |
| Abstract: | Lymphocystis disease virus-Chinese isolate(LCDV-cn) is the causatice agent of lymphocystis disease in paralichthys olivaceus,causing a huge loss in flounder aquaculture.Some gene sequences of LCDV-cn has been acquired by PCR,cloning and sequencing.Those sequences were consistent with LCDV-C.Found through comparative analysis,137 ORF is LCDV-cn’s own unique genes without homology with LCDV-1 and other iridovirus.LCDV-cn genome was analyzed by bioinformatics methods.In proteins encoded by LCDV-cn’s unique genes,some protein was homologous with fish protein;some protein was related to DNA duplication;and some protein contained transmenmbrane domain,etc.It is likely that LCDV-cn’s unique genes influence its virulence and host range.This study can form the foundation for in-depth researches,such as mechanism of LCDV-cn’s pathopoiesia and infection,prevention and treatment of lymphocystis disease,and so on. |
| |
| Keywords: | Lymphocystis disease virus-Chinese isolate(LCDV-cn) Comparative genomics Bioinformatics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
