包埋脱水法冷冻保存裙带菜配子体克隆的研究

将裙带菜配子体克隆包埋在含3%褐藻酸钙的胶球中,研究了预培养中的蔗糖浓度和培养时间,脱水过程中的脱水速率和胶球含水量以及化冻速度对配子体克隆的存活率和发育能力的影响,结果表明含裙带菜配子体克隆的胶球在0.3 mol/dm3的蔗糖培养基中培养9 h可以获得较高的存活率;胶球最适平均脱水速率为含水量的5%每小时,最适含水量为25%;较快的化冻速度可以显著提高配子体克隆的存活率,在优化条件下存活率可高达66%;经过冷冻保存的裙带菜配子体克隆在适合发育的条件下能够正常形成孢子体.

Cryopreservation of clonal gametophytes of Undaria pinnatifida by encapsulation-dehydration

Clonal gametophytes of Undaria pinnatifida were encapsulated in 3% calcium alginate beads. The main factors influencing the viability and the development ability of clonal gametophytes, such as sucrose concentration, preculture time, dehydration rate, water content of beads and thawing rate were studied. The results show that the high viability can be obtained when alginate beads are precultured for 9 h in the medium containing 0.3 mol/dm~3 sucrose. The optimum average dehydration rate of beads is reduction of 5% water content per hour and the optimum water content of beads is 25%. Fast rate of thawing can significantly improve the viability of clonal gametophytes. Under optimum conditions, the cryopreservation viability of clonal gametophytes can reach 66% at the most. After cryopreservation, the clonal gametophytes can produce the normal sporophytes in the conditions suitable for development.