海洋微藻高度不饱和脂肪酸的硏究 Ⅱ.环境因子对球等鞭金藻3011中高度不饱和脂肪酸含量的影响

微藻在不同培养条件下,藻体中脂类和高度不饱和脂肪酸(PUFA)的含量及种类可发生很大变化。因此,研究不同培养条件对其含量的影响,以期寻找既能促进藻类较快生长，又能使其体内PUFA含量较高的培养条件,对于促进水产养殖的发展,乃至使其成为生产EPA和DHA的工业原料,无疑都具有重要的意义。 在系列研究I中我们报告了我国沿海水产养殖中常用的7种微藻的脂类和脂肪酸组成,在其基础上,我们选择了脂肪和DHA含量比较高的球等鞭金藻3011(Isochrysis galbana),用不同的营养液、并在不同的生长期、温度、盐度等条件下,对其进行培养,分析藻体中PUFA含量的变化,以便更好地提高其营养价值和利用率,同时探讨用其作为生产PUFA的工业原料的可能性。

STUDIES ON POLYUNSATURATED FATTY ACIDS OF MARINE MICROALGAE I. EFFECTS OF CULTURE CONDITIONS ON THE CONTENTS OF FATTY ACIDS OF ISOCHARYSIS GALBANA 3011

This study of the effects of culture conditions (season, salinity, growth period, culture medium ) on lipid and fatty acid composition of Isochrysis galbana 3011 showed: (1) Total lipid and DHA contents in the algae increased with decreasing temperature; (2)Too high and too low salinity slowed production of total lipid and DHA, suitable salinity was 2.5%-3.0%; (3) Total lipid and DHA contents in the exponential growth period were much higher than that in the static period; (4) Total lipid and DHA content of the algae growing in different culture medium varied very much, and the optimal medium for growth of the algae was:N, 2.0 mmol/L; P, 0.05mmol/L: Fe, 0.002 mmol/L; Si, 0 mmol/L; and C, 0 mmol/L.