| Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis |
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| 作者姓名： | 张晓军 李富花 相建海 |
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| 作者单位： | Institute of Oceanology，Institute of Oceanology，Institute of Oceanology Chinese Academy of Sciences，Qingdao 266071，China，Chinese Academy of Sciences，Qingdao 266071，China，Chinese Academy of Sciences，Qingdao 266071，China |
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| 基金项目： | ContributionNo.440 7fromtheInstituteofOceanology,ChineseAcademyofSciences.ThisworkwassupportedbyProjectunderMajorStateBasicDevelopmentProgram (G1 9990 12009)andKnowledgeInnovationProgramoftheChineseAcademyofSciences(KZCX 2 2 1 1) |
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| 摘 要： | INTRODUCTIONTriploidanimalsareusefulbecausetheyaresterile ,characterizedbypositivegrowthinthere productiveseason ,usefulfortesting ,forintroductionofnon nativespecies ,andforprotectionofde velopedstrains.Theinductionoftriploidyhadbeenreportedinmanyaquaculturespecies .Triploidshavebeensuccessfullyinducedinshrimpusingtemperatureshockorchemicalshock (CB ,6 DMAP)in 4species :Sicyoniaingentis (Xiangetal.,1 991 ) ,Fenneropenaeuschinensis (Xiangetal.,1 992 ;Daietal.,1 993;Baoetal.,1 993;Li…
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| 关 键 词： | 三倍体 染色体行为 热诱变 荧光显微镜 动物遗传学 |
Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis |
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| ZHANG Xiaojun,LI Fuhua,XIANG Jianhai.Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis[J].Chinese Journal of Oceanology and Limnology,2003,21(3):222-228. |
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| Authors: | ZHANG Xiaojun LI Fuhua XIANG Jianhai |
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| Abstract: | Triploidy was induced in Chinese shrimp Fenneropenaeus chinensis by 30 + 0.5 ℃ heat shock treatment (initiated at 20 min after fertilization) for 10 min to inhibit the release of PB2 at 18.0℃ . The highest triploid rate obtained was 84.5% in nauplius stage. The effect of heat shock treatment on meiosis and cleavage of eggs was investigated in this work aimed to establish ef ficient procedures for triploid induction and to gain understanding of the mechanism of triploid production. Three pronuclei that could be observed in the treated eggs under fluorescence microscope developed into triploid embryos. Some abnormal chromosome behavior was observed in heat shocked eggs. |
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| Keywords: | triploid induction chromosome behavior heat shock Fenneropenaeus chinensis |
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