| In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus |
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| 作者姓名： | SU Feng ZHANG Shicui YANG Ming LI Hongyan |
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| 作者单位： | [1]Department of Bioengineering ＆ Pharmaceutics, Qingdao University of Science ＆ Technology, Qingdao 266042, China [2]Department of Marine Biology, Ocean University of China, Qingdao 266003, China |
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| 基金项目： | The authors thank Ms.Pang Qiuxiang and Mr.Wang Yongjun for their technical assistances.This work was supported by the Scientific Research Fund of Qingdso University of Science and Technology. |
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| 摘 要： | The in vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cell line, derived from the gill of flounder Paralichthys olizaceus, was tested by the three widely used endpoint bioassays-neutral red （NR） assay, tetrazolium （MTT） assay and cell protein assay. It was found that acetamiprid was increasingly toxic to FG cells at concentrations of 1 μg/cm^3 or above, and the inhibitory concentration 50% values for NR, MTF, and cell protein assays were 38.38, 36.27 and 32.03 μg/cm^3, respectively. This appeared to be the first report on the in vitro cytotoxicity of acetamiprid to non-mammalian vertebrate cells. Ultrastructural examination revealed that for the cells exposed to 60 μg/cm^3 acetamiprid for 48 h, their mitochondria were severely damaged with the cristae swelled up or disrupted, while their nuclei and rough endoplasmic reticlum （RER） appeared to be still normal. This suggests that mitochondria are possibly the primary target of acetamiprid.
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| 关 键 词： | 新烟碱类杀虫剂 啶虫脒 比目鱼腮PG细胞系 急性细胞毒性 |
| 收稿时间： | 2006-02-28 |
| 修稿时间： | 2006-06-27 |
In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus |
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| SU Feng,ZHANG Shicui,YANG Ming,LI Hongyan.In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus[J].Acta Oceanologica Sinica,2006,25(5):135-140. |
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| Authors: | SU Feng ZHANG Shicui YANG Ming and LI Hongyan |
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| Institution: | 1.Department of Bioengineering & Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China;Department of Marine Biology, Ocean University of China, Qingdao 266003, China2.Department of Marine Biology, Ocean University of China, Qingdao 266003, China |
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| Abstract: | The in vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cell line, derived from the gill of flounder Paralichthys olivaceus, was tested by the three widely used endpoint bioassays-neutral red (NR) assay, tetrazolium (MTT) assay and cell protein assay.It was found that acetamiprid was increasingly toxic to FG cells at concentrations of 1 μg/cm3 or above, and the inhibitory concentration 50% values for NR, MTT, and cell protein assays were 38.38, 36.27 and 32.03 μg/cm3, respectively.This appeared to be the first report on the in vitro cytotoxicity of acetamiprid to non-mammalian vertebrate cells.Ultrastructural examination revealed that for the cells exposed to 60 μg/cm3 acetamiprid for 48 h, their mitochondria were severely damaged with the cristae swelled up or disrupted, while their nuclei and rough endoplasmic reticlum (RER) appeared to be still normal.This suggests that mitochondria are possibly the primary target of acetamiprid. |
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| Keywords: | acetamiprid cell line cytotoxicity flounder |
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