| 低温脂肪酶基因在巴斯德毕赤酵母中的高效表达 |
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| 引用本文： | 王永杰,沈继红,丛柏林,林学政,黄晓航.低温脂肪酶基因在巴斯德毕赤酵母中的高效表达[J].极地研究,2011,23(4):283-288. |
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| 作者姓名： | 王永杰 沈继红 丛柏林 林学政 黄晓航 |
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| 作者单位： | 1. 国家海洋局第一海洋研究所;
2. 海洋生物活性物质国家海洋局重点实验室 |
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| 基金项目： | 863计划“极地特殊海洋微生物资源利用的关键技术研究(2007AA091905)”资助 |
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| 摘 要： | 通过PCR技术扩增得到南极适冷菌低温脂肪酶基因lip-948,将其与表达载体pPIC9K连接后转化巴斯德毕赤酵母GS115,通过筛选得到高效表达低温脂肪酶的酵母菌株,该菌株发酵48 h后上清液中脂肪酶活力达到27.5 U·mL-1,普遍高于其他细菌低温脂肪酶在毕赤酵母中的表达活性.
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| 关 键 词： | 低温脂肪酶表达载体pPIC9K 巴斯德毕赤酵母高效表达 |
| 收稿时间： | 2011-02-28 |
HIGH-LEVEL EXPRESSION OF A COLD-ADAPTIVE LIPASE GENE IN PICHIA PASTORIS |
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| Wang Yongjie,Shen Jihong,Cong Bailin,Lin Xuezheng,Huang Xiaohang.HIGH-LEVEL EXPRESSION OF A COLD-ADAPTIVE LIPASE GENE IN PICHIA PASTORIS[J].Chinese Journal of Polar Research,2011,23(4):283-288. |
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| Authors: | Wang Yongjie Shen Jihong Cong Bailin Lin Xuezheng Huang Xiaohang |
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| Institution: | 1. First Institute of Oceanography, SOA;
2. Key Lab of Marine Bioactive Substances, SOA |
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| Abstract: | A cold-adaptive lipase gene lip-948 from Antarctic psychrophilic bacteria was ligated into the expression vector pPIC9K, which was transformed into yeast GS115 subsequently. High-level express yeast was obtained . Lipase activity of the supernant reached 27.5 U/mL after 48 h fermentation, which is higher than most of other low-temperature bacteria lipase genes expressed in Pichia pastoris. |
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| Keywords: | low-temperature lipase pPIC9K Pichia pastoris high-level expression |
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