| 仿刺参(Apostichopus japonicus)和海地瓜(Acaudina leucoprocta)体壁多肽的响应面法酶解和N末端测序 |
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| 引用本文： | 李妍妍,戴娟,胡玲萍,江振洲,尚靖,张陆勇.仿刺参(Apostichopus japonicus)和海地瓜(Acaudina leucoprocta)体壁多肽的响应面法酶解和N末端测序[J].海洋与湖沼,2015,46(3):620-627. |
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| 作者姓名： | 李妍妍 戴娟 胡玲萍 江振洲 尚靖 张陆勇 |
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| 作者单位： | 国家南京新药筛选中心 中国药科大学 南京 210009,宁波大学海洋学院 宁波 315211,宁波大学海洋学院 宁波 315211,国家南京新药筛选中心 中国药科大学 南京 210009,国家南京新药筛选中心 中国药科大学 南京 210009,国家南京新药筛选中心 中国药科大学 南京 210009 |
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| 基金项目： | 国家科技星火计划资助项目, 2010GA701063 号; 国家农业科技成果转化资金资助项目, 2007GB2C220359 号; 浙江省重大科技专项, 2008C02009 号。 |
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| 摘 要： | 为充分开发利用海参资源,优化海参体壁酶解工艺,鉴定海参多肽,本文以水解度为指标,设计了3因素(时间、温度和加酶量)3水平的响应面实验,得到海参水解的最优条件是:仿刺参(Apostichopus japonicus)体壁在加酶量1.97%、温度55.7°C、水解136.8min的条件下,水解度为83.39%;海地瓜(Acaudina leucoprocta)体壁在加酶量1.70%、温度55.4°C、水解115.6min的条件下,水解度为63.68%。之后通过使用超滤膜、反相高效液相色谱(RP-HPLC)、基质辅助激光解析电离飞行时间质谱(MALDI-TOF)技术和蛋白测序仪等分析手段从水解产物中分离鉴定出4种多肽。其中,经N端序列鉴定出仿刺参多肽S1、S2氨基酸残基序列分别为Gly-Pro-Val-Gly-Ala-Ser-Gly-Pro-Gln-Gly-ProGln-Gly-Pro-Gln-Gly-Leu-Ser-Ala-Leu和Trp-Pro-Pro-Gly-Asn-Ser-Gly-Ile-Gln-Gly。海地瓜多肽A1和A2氨基酸残基序列分别为Gly-Ala-Asn-Gly-Asn和Trp-Leu-Pro-Gly-Asp-Thr-Gly-Pro-Gln-GlyVal-Thr-Gly-Pro-Val-Gly-Pro-Ala-Gly。
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| 关 键 词： | 仿刺参 海地瓜 响应面 多肽分离纯化 N 端测序 |
| 收稿时间： | 2014/11/2 0:00:00 |
| 修稿时间： | 2014/12/19 0:00:00 |
ENZYMATIC HYDROLYSIS TECHNOLOGY OF APOSTICHOPUS JAPONICUS AND ACAUDINA LEUCOPROCTA BASED ON RESPONSE SURFACE METHODOLOGY AND N-TERMINAL AMINO ACID SEQUENCING |
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| LI Yan-Yan,DAI Juan,HU Ling-Ping,JIANG Zhen-Zhou,SHANG Jing and ZHANG Lu-Yong.ENZYMATIC HYDROLYSIS TECHNOLOGY OF APOSTICHOPUS JAPONICUS AND ACAUDINA LEUCOPROCTA BASED ON RESPONSE SURFACE METHODOLOGY AND N-TERMINAL AMINO ACID SEQUENCING[J].Oceanologia Et Limnologia Sinica,2015,46(3):620-627. |
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| Authors: | LI Yan-Yan DAI Juan HU Ling-Ping JIANG Zhen-Zhou SHANG Jing and ZHANG Lu-Yong |
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| Institution: | Center for Drug Screening, National Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China,Center for Drug Screening, National Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, China,Center for Drug Screening, National Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, China and Center for Drug Screening, National Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, China |
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| Abstract: | |
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| Keywords: | Apostichopus japonicas Acaudina leucoprocta response surface methodology separation and purification of peptides N-terminal sequence |
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