| 龙须菜UDP-葡萄糖焦磷酸化酶基因的克隆及其表达与琼胶产量的关系 |
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| 引用本文： | 张杨,隋正红,丁弘叶,仲洁.龙须菜UDP-葡萄糖焦磷酸化酶基因的克隆及其表达与琼胶产量的关系[J].中国海洋大学学报(自然科学版),2011(Z1):80-86. |
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| 作者姓名： | 张杨 隋正红 丁弘叶 仲洁 |
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| 作者单位： | 中国海洋大学海洋生命学院海洋生物遗传育种教育部重点实验室; |
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| 基金项目： | 国家自然科学基金项目(30771647); 国家高技术研究发展规划项目(2006AA10A413)资助 |
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| 摘 要： | 对红藻龙须菜(Gracilarialei maneiformis)UDP葡萄糖焦磷酸化酶(UGPase)基因的表达与不同藻株中藻体琼胶产量之间的相关性进行研究。通过PCR扩增的方法得到了龙须菜UGPase的基因序列。该基因cDNA序列的全长为2 200 bp,开放阅读框1 485 bp,编码494个氨基酸;龙须菜UGPase基因的氨基酸序列与其它物种的UGPase氨基酸序列相似性较高;该基因是单拷贝的,缺乏内含子;具有特殊的加尾信号。利用荧光实时定量PCR的方法检测UGPase基因相对表达量。结果表明,该基因的表达与琼胶含量存在显著相关性,即在高琼胶组藻株中的表达量显著高于低琼胶组藻株,对琼胶含量高低具有指示作用,显示了在产业化应用中的潜能。
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| 关 键 词： | 龙须菜 UDP-葡萄糖焦磷酸化酶 琼胶 实时定量PCR |
Cloning and Analysis of the UDP-Glucose Pyrophosphorylase(UGPase) Gene of Gracilaria leimaneiformis(Rhodophyta) and Correlation Between Gene Expression and Agar Synthesis |
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| ZHANG Yang,SUI Zheng-Hong,DING Hong-Ye,ZHONG Jie.Cloning and Analysis of the UDP-Glucose Pyrophosphorylase(UGPase) Gene of Gracilaria leimaneiformis(Rhodophyta) and Correlation Between Gene Expression and Agar Synthesis[J].Periodical of Ocean University of China,2011(Z1):80-86. |
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| Authors: | ZHANG Yang SUI Zheng-Hong DING Hong-Ye ZHONG Jie |
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| Institution: | ZHANG Yang,SUI Zheng-Hong,DING Hong-Ye,ZHONG Jie(Key Laboratory of Marine Genetics and Breeding Ministry of Education,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | The purpose of the study is to investigate the relationship between gene expression level of UDP-glucose pyrophosphorylase(UGPase) and the agar content in red algae Gracilaria leimaneiformis.PCR was employed to obtain the gene sequence,which gave out a cDNA of 2200 bp,coding for 494 amino acids,involving 1485 bp of ORF.The deduced amino acid sequence showed significant similarity to the UGPase sequences of other species.The gene is single-copy,devoiding of introns,and has special polyA tailing signal.Real t... |
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| Keywords: | Gracilariopsis lemaneiformis UDP-glucose pyrophosphorylase agar real-time quantitive PCR |
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