黄海春季和秋季中华哲水蚤脂类含量及组成分析

脂类物质在哲水蚤的休眠、繁殖以及个体发育过程中发挥着重要作用,通过分析2010年11月(秋季)和2011年4月(春季)黄海中华哲水蚤Calanus sinicus的脂类组成,对脂类各组分的生理功能进行了初步探讨。结果表明,春季、秋季中华哲水蚤雌体脂类平均含量占干质量的10.6%和12%,C5期个体脂类含量偏高,占到干质量的17%。中华哲水蚤所含脂类主要由蜡脂和磷脂组成,两种脂类约占总脂的90%,三酰甘油含量约占总脂的6%。结合种群结构对脂类的生理作用进行了初步探讨,发现:(1)蜡脂作为主要的能量储存方式,其含量变化反映出不同发育期、不同季节中华哲水蚤的能量需求变化;(2)磷脂作为结构性脂类在中华哲水蚤个体生长与生殖过程中具有重要作用;(3)三脂酰甘油在中华哲水蚤中的含量不高,但是可能在脂类转化利用过程中发挥作用。

Lipid content and composition of Calanus sinicus in the Yellow Sea in spring and autumn

Lipid plays an important role in reproduction, food scarcity and diapause of zooplankton. The present research studied the role of lipids in Calanus sinicus, and samples were collected from the Southern Yellow Sea in autumn (November, 2010) and spring (April, 2011). The results show that the total lipid content of female C. sinicus was about 10.6%-12% of dry body mass, while C5 stage in spring had a higher percentage, about 17% of dry mass. The major components in lipid classes were wax ester (WE) and phospholipid (PL), and they together contributed about 90% to total lipid, while triglyceride (TAG) accounted for only 6%. The role of different lipid classes was discussed as follows: (1) Wax ester served as an important energy deposit. The fluctuated WE content of C. sinicus suggested the different strategies in energy needs and utilization of different development stages and seasons. (2) The coincidence of active reproduction and high content of PL in female indicated that PL plays an important role in the egg production of C. sinicus. (3) The composition of TAG was relatively lower than that of WE and PL in C. sinicus, but it might work in the transformation of different lipid classes.