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| 杜氏盐藻叶绿体ATP合酶α亚单位基因启动子的克隆及初步功能分析 | |
| 引用本文： | 谷辉辉,冯书营,潘卫东,李 杰,薛乐勋.杜氏盐藻叶绿体ATP合酶α亚单位基因启动子的克隆及初步功能分析[J].海洋科学,2011,35(9):18-23. |
| 作者姓名： | 谷辉辉 冯书营 潘卫东 李 杰 薛乐勋 |
| 作者单位： | 1. 郑州大学生物工程系,河南郑州,450001 2. 河南科技大学医学技术与工程学院,河南洛阳,471003 3. 郑州大学基础医学院,河南郑州,450052 |
| 基金项目： | 科技部国际科技合作项目(2007DFA01240); 国家自然科学基金项目(30700014) |
| 摘 要： | 采用DraⅠ，EcoRⅤ，PvuⅡ，ScaⅠ，SmaⅠ和StuⅠ六种内切酶消化杜氏盐藻（Dunaliella salina）叶绿体基因组DNA，与相应DNA接头连接，构建成无载体连接的盐藻叶绿体Genome Walker DNA文库。利用长距离PCR（long distance-polymerase chain rea... |
| 关 键 词： | 杜氏盐藻(Dunaliella salina) 叶绿体转化 ATP合酶α亚单位基因启动子 |
| 收稿时间： | 2010/7/10 0:00:00 |
| 修稿时间： | 2010/11/5 0:00:00 |
Cloning and preliminary functional analysis of ATPase alpha subunit gene promoter from Dunaliella salina |
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| GU Hui-hui,FENG Shu-ying,PAN Wei-dong,LI Jie,XUE Le-xun.Cloning and preliminary functional analysis of ATPase alpha subunit gene promoter from Dunaliella salina[J].Marine Sciences,2011,35(9):18-23. | |
| Authors: | GU Hui-hui FENG Shu-ying PAN Wei-dong LI Jie XUE Le-xun |
| Institution: | GU Hui-hui1,FENG Shu-ying2,PAN Wei-dong3,LI Jie1,XUE Le-xun1(1.Department of bioengineering,Zhengzhou University,Zhengzhou 450001,China,2.College of Engineering and Medical Technology of Henan Science and Technology University,Luoyang 471003,3.Medical College,Zhengzhou 450052,China) |
| Abstract: | To clone and identify the function of the promoter of the ATPase alpha subunit (atpA) gene from Dunaliella salina, a chloroplast GenomeWalker DNA Library from D. salina was constructed, from which the 1 347 bp upstream fragment of atpA gene was cloned by long distance-polymerase chain reaction (LD-PCR). According to the positions of the different promoter elements predicted by bioinformatics tools, 5'-deleted series primers were designed to amplify different promoter fragments. Series 5'-deleted expression vector containing the GFP gene was constructed and then transformed to cells of D. salina by particle bombardment. The transformed cells with p1000 recombinant vector produced green fluorescence, but not in other transformation groups, suggesting that the cloned region had the activity of the promoter and may drive the effective expression of the GFP gene in D. salina. Our results have layed a foundation for the chloroplast transformation system of D. salina. |
| Keywords: | Dunaliella salina chloroplast transformation ATPase alpha subunit gene promoter |
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