| 日本蟳(Charybdis japonica)微卫星位点的分离及遗传多样性分析 |
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| 引用本文： | 叶 茂,申 望,王日昕,郑兆祥,石 戈.日本蟳(Charybdis japonica)微卫星位点的分离及遗传多样性分析[J].海洋与湖沼,2011,42(1):86-93. |
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| 作者姓名： | 叶 茂 申 望 王日昕 郑兆祥 石 戈 |
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| 作者单位： | 浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室,舟山,316004 |
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| 基金项目： | 海洋渔业科学与技术浙江省重中之重学科开放课题资助, 20100205 号; 浙江省自然科学基金项目, Y3080292 号 |
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| 摘 要： | 采用磁珠富集法,以生物素标记的(cA)12寡核苷酸为探针,构建了日本蟳基因组微卫星富集文库.通过PCR法从文库中共筛选出89个微卫星位点,其中完全型(perfect)共75个,占84.27%;不完全型(imperfect)12个,占13.48%;复合型(compound)2个,占2.25%.根据微卫星位点的侧翼序列,设...
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| 关 键 词： | 日本蟳 微卫星 磁珠富集法 遗传多样性 |
| 收稿时间： | 2010/5/17 0:00:00 |
| 修稿时间： | 2010/6/30 0:00:00 |
ISOLATION AND GENETIC DIVERSITY OF MICROSATELLITE LOCI IN ASIAN PADDLE CRAB CHARYBDIS JAPONICA |
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| YE Mao,SHEN Wang,WANG Ri-Xin,ZHENG Zhao-Xiang and SHI Ge.ISOLATION AND GENETIC DIVERSITY OF MICROSATELLITE LOCI IN ASIAN PADDLE CRAB CHARYBDIS JAPONICA[J].Oceanologia Et Limnologia Sinica,2011,42(1):86-93. |
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| Authors: | YE Mao SHEN Wang WANG Ri-Xin ZHENG Zhao-Xiang and SHI Ge |
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| Institution: | Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University;Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University |
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| Abstract: | To isolate and identify polymorphic microsatellite markers which would be suitable for evaluating the genetic diversity and population genetic structure of Charybdis japonica, an enriched microsatellite library of C. japonica had been constructed by a magnetic-bead enrichment method used biotinylated (CA)12 probe. Screening the enriched microsatellite library by PCR technique, 89 microsatellite sequences were obtained. Among these microsatellite sequences, the numbers of perfect, imperfect and compound sequences were 75 (84.27%), 12 (13.48%) and 2 (2.25%), respectively. To analyze genomic DNA diversity of C. japonica, 9 pairs of polymorphic primers were designed and labeled by fluorophore. Allelic variation and other characteristics at these loci were examined in C. japonica population collected from Zhoushan sea area, China. The number of alleles per locus ranged from 12 to 25; the observed and expected heterozygosity varied from 0.5806 to 0.9032 and from 0.8383 to 0.9449, respectively; polymorphism information content (PIC) ranged form 0.8092 to 0.9259. These results showed that these microsatellite loci would be useful for studies of population genetics, stock management and other relevant research in C. japonica. |
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| Keywords: | Charybdis japonica Microsatellite Magnetic-bead enrichment method Genetic diversity |
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