| 海水中-氧化氮的化学发光法测定 |
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| 引用本文： | 丰卫华,刘春颖,杨桂朋.海水中-氧化氮的化学发光法测定[J].海洋学报,2011,33(6):93-99. |
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| 作者姓名： | 丰卫华 刘春颖 杨桂朋 |
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| 作者单位： | 中国海洋大学 化学化工学院 海洋化学理论与工程技术教育部重点实验室,山东 青岛266100 |
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| 基金项目： | 国家自然科学基金 (41176062,40706040,40376022);山东省中青年科学家科研奖励基金(2007BS08015);山东省自然科学基金(Y2008E04);教育部"长江学者"奖励计划;山东省"泰山学者"建设工程资助项目。 |
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| 摘 要： | 基于鲁米诺-H2O2混合溶液作为发光液与海水中NO发生反应产生发光信号的原理,提出了一种测定海水中痕量NO的化学发光法.确定了化学发光法测定NO的最佳条件:pH9.2、浓度1×10-2 mol/dm3的碳酸盐为缓冲体系,鲁米诺浓度为8.4×10-5 mol/dm3,H2O2浓度为1×10-2 mol/dm3,测定温度为...
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| 关 键 词： | 一氧化氮(NO) 化学发光法 海水 鲁米诺-H2O2 胶州湾 |
| 收稿时间： | 2010/11/1 0:00:00 |
| 修稿时间： | 5/8/2011 12:00:00 AM |
Chemiluminescence detection of nitric oxide in seawater |
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| FENG Wei-hu,LIU Chun-ying and YANG Gui-peng.Chemiluminescence detection of nitric oxide in seawater[J].Acta Oceanologica Sinica (in Chinese),2011,33(6):93-99. |
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| Authors: | FENG Wei-hu LIU Chun-ying and YANG Gui-peng |
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| Institution: | Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China |
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| Abstract: | A chemiluminescence method measuring the concentration of NO in seawater was established in this paper on the base of a reaction between the mixed solution of luminol-H2O2, the chemiluminescence probe, and NO, in which the chemiluminescence signal was emitted. The optimal results in the determination of NO concentration were under the conditions of a pH 9.2 buffer system with 1×10-2 mol/dm3 carbonate, 8.4×10-5 mol/dm3 luminol, 1×10-2 mol/dm3 H2O2, and under 20 ℃ and 1×10-3 mol/dm3 EDTA as a optimal masking agent. Under these optimal conditions, there was a significant linear relationship between the NO concentration and the peak area of chemiluminescence signal in the range of NO concentration from 1.4×10-12 to 9.8×10-10 mol/ dm3 (R=0.997, P <0.000 1). The detection limit for NO was 1.4×10-12 mol/dm3 (S/N). In 7 times of parallel determination to 1.2×10-11 mol/dm3NO aqueous solution, the relative standard deviation (RSD) was 13.6%. The NO concentration in the surface seawater of Jiaozhou Bay, China was successfully determined using this method and the results indicated that the concentrations of NO ranged from undetected to 7.1×10-11 mol/ dm3 with an average of (1.6±1.8)×10-11 mol/dm3. |
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| Keywords: | nitric oxide (NO) chemiluminescence seawater luminol-H2O2 Jiaozhou Bay |
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