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GENETIC DIFFERENTIATION WITHIN ERIOCHOIR SINENSIS(MILNE,EDWARDS) REVEALED BY ALLOZYME AND RAPD
Authors:CUI Zhao-xia  XIANG Jian-hai  SONG Lin-sheng  ZHOU Ling-hua  SHI Wei-liang
Abstract:We analyzed 17 allozymes and 20 primers in order to detect the genetic differentiation between commercial populations (Changjiang River, Liaohe River) of Eriochoir sinensis . Ten allozymes (LDH, MDH, ME, IDH, EST, ALP, AAT, CTL, POD, SOD) showed 21 loci by vertically discontinuos buffer system polyacrylamide gel electrophoresis. RAPD profiles generated by 12 ten base primers showed 63 loci. The percentage of polymorphic loci and the expected heterozygosity obtained by using allozyme analysis were lower than those obtained by RAPD. The index of similarity between these two populations were 0.955 and 0.932 as revealed by allozyme analysis and RAPD technology. There was gene flow between the above populations.
Keywords:Eriocheir sinensis    allozyme  RAPD  genetic differentiation
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