Studies on color type variants from mutagenized protoplasts ofPorphyra haitanensis Chang et Zheng &P. Yezoensis ueda (rhodophycease) |
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Authors: | Yan Xinghong |
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Institution: | (1) Department of Aquaculture, Shanghai Fisheries University, 200090 shanghai |
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Abstract: | Isolated protoplasts from thalli ofPorphyra haitanensis andPorphyra yezoensis were treated with colchicine or irradiated by ultraviolet (UV). Several types of color variants were observed among the protoplast
offspring. After treatment with colchicine: (1) 0.04–0.09% of red type variants inP. haitanensis were obtained; (2) The rate of red type variants and the variegated chimeral thalli composed of red type and wild type of
sectors were 0.31–1.11% inP. yezoensis. After irradiation with UV: (1) 3.5–10.5% of red type variants inP. yezoensis were obtained: (2) 0.5–2.0% of red type variants and the variegated chimeral thalli composed of red type and wild type of
sectors were obtained inP. haitanensis. Colchicine and UV’s mutangenic effects onP. yezoensis protoplasts were stronger than those onP. haitanensis protoplasts. The most efficient concentration of colchicine was 0.05%. The optimal length of UV-radiation was 1/2 min (radiation
distance 5 cm). The red type variants induced by colchicine treatment grew faster than the wild type thalli. The clones of
vegetative propagation from protoplasts of red type variants were still red type thalli. The red type variants will be good
materials for genetic studies and improvement ofPorphyra strains. |
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Keywords: | Porphyra protoplast color type variant variegated chimeral thallus |
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