DNA tracers with information capacity and high detection sensitivity tested in groundwater studies |
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| Authors: | I. H. Sabir Jacob Torgersen Sylvi Haldorsen Peter Aleström |
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| Affiliation: | Geology and Water Section, Department of Soil and Water Sciences, Agricultural University of Norway, P.O. Box 5028, N-1432 ?s, Norway Fax: +47-64-948211 e-mail: iftikhar.sabir@ijvf.nlh.no, NO
Department of Biochemistry, Physiology, and Nutrition, Norwegian College of Veterinary Medicine, P.O. Box 8146 Dep. N-0033 Oslo, Norway, NO
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| Abstract: | In order to investigate the usefulness of unique synthetic DNA tracers in groundwater, a field experiment was conducted in Norway. DNA tracers and a sodium-chloride tracer were injected into an aquifer. The transport of DNA molecules was interpreted by comparing with the plume of chloride ions under forced-gradient steady-state flow conditions. Spatial concentration moments described the migration of conservative tracers. Mobility and migration of DNA in groundwater demonstrate that DNA tracers can be detected by using the polymerase chain reaction (PCR) and DNA sequence analysis. The results indicate that DNA tracers can be valuable tools as tracers in groundwater investigations. Received, June 1997 / Revised, July 1998, December 1998 / Accepted, January 1999 |
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| Keywords: | DNA tracer tests solute transport groundwater flow Norway |
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