AFLP技术在笛鲷的仔鱼鉴定及其分类学上的研究

以南沙群岛采获的勒氏笛鲷(Lutjanus russelli)、黄笛鲷(Lutjanus lutjanus)、红笛鲷(Lut janus sanguineus)、紫红笛鲷(Lutjanus argentimaculatus)、金焰笛鲷(Lutjanus fulviflamma)、线纹笛鲷(Lutjanus lineolatus)、画眉笛鲷(Lutjanus vitta)、马拉巴笛鲷(Lutjanus malabarius)、驼背笛鲷(Lutjanus gibbus)、约氏笛鲷(Lutjanus johni)、金带笛鲷(Lutjanus vaigiensis)11种笛鲷属的后期仔鱼为材料, 进行了基因组DNA的AFLP(扩增片段长度多态性)研究,通过笛鲷仔鱼和成鱼的AFLP电泳图谱的比较分析,将这11种笛鲷属的仔鱼成功分开.研究表明,最适合的AFLP引物组合是E+AGC/M+CAA,由这11种笛鲷共扩增出132 AFLP位点,每种笛鲷的AFLP条带数为43~69,在这11种笛鲷中,共享的AFLP条带数仅为7,同一种笛鲷仔鱼和成鱼AFLP遗传相似度在90%以上.根据这11种笛鲷的Nei遗传距离对Neighboring系统进行进化分析,并且与传统的笛鲷形态分类进行了比较.分子生物学可以阐释笛鲷属鱼类的系统进化和遗传亲缘关系,对传统形态分类学加以完善和补充.

Studies on the larval identification and taxonomy of Lutjanus by AFLP technology

The Nansha Coral Reefs (also called Spratly Archipelago) water area, which is located in the South China Sea, is the main habitat and spawn area for the species of Lutjanus in China. The larvae of many closely related species, such as those of the genus Lutjanus, are different to be distinguished morphologically. A PCR-based fingerprinting technology called amplified fragment length polymorphism(AFLP)was used in the characterization and identification of 11 species of the genus Lutjanus sampled from the Nansha Coral Reefs. The optimal AFLP pattern was obtained with primer combination of E+ AGC/ M + CAA selective nucleotides. Totally, 132 AFLP loci were found from these species and the number of AFLP bands for each species varied from 43 to 69. More than 90% of AFLP bands were species-specific and only 7 markers were fixed to all species examined. All larvae were successfully identified to 11 Lutjanus species by comparing AFLP fingerprints of larvae with that of adults. Moreover, the phylogenetic analysis (Neighboring) of species relationship based on the AFLP data was compared with the current morphological taxonomic data. It suggests that AFLP analysis is a reliable method for the larval identification and taxonomy of the genus Lutjanus.