吸附-交联法固定胰蛋白酶及在澄清啤酒中的应用

胰蛋白酶的固定化采用吸附-交联法.在单因素试验基础上,通过正交试验,获得了胰蛋白酶固定化的最佳条件酶与载体体积比为21,吸附时间为10 h,pH=4.5,缓冲液浓度为0.05 mol/L,戊二醛的质量分数为0.1%.此条件下制得的磁性酶活力回收在50%左右,相对酶活力达74%,该磁性酶对啤酒澄清防止冷浑浊有明显效果.

Study on Immobilization of Trypsin by Absorbing and Crosslinking and Its Application in Beer Clarification

Trypsin is immobilized on magnetic colloid through absorbing and crosslinking. On the basis of single factor experiments,the best condition of the immobilization of trypsin can be found through orthogornal experiments:The ration of enzyme to carriers is two to one ,absorption time 10 hours,pH=4.5,concentration of the buffer solution 0.05 mol/L,quality fraction of the glutaril dialdehyde, 0.1%.The activity recovery of immobilized enzyme is around 50% and relative enzyme activity 74% under these conditions. MIE can clarify beer and prevent cold turbid.