实验微粒饲料中廿碳五烯酸(EPA)与廿碳四烯酸(AA)的比例对牙鲆仔稚鱼生长、存活的影响

制备了4种n-3系列高度不饱和脂肪酸（n-3HUFA）含量相等，廿二碳六烯酸（DHA）含量相等，廿碳五烯酸（EPA）含量亦相等，廿碳五烯酸与廿碳四烯酸（AA）的比例分别为5／1，2／1，1／1和0．4／1的实验微粒饲料。探讨了EPA与AA的不同比例对牙鲆仔稚鱼生长、存活、体内相关成分以及对外部压力耐受性的影响。26d的养殖试验结果表明，当牙鲆仔稚鱼实验微粒饲料中EPA与AA的比例为2／1时，牙鲆仔稚鱼的生长、存活达到最佳。因此，在该试验条件下，牙鲆仔稚鱼实验微粒饲料中EPA与AA的最佳比例为2／1。养殖试验结束后，4组试验稚鱼体内脂肪酸的分析测定结果表明，稚鱼体内AA的含量，随着微粒饲料中AA含量的增高而增大。

Effects of different EPA/AA ratios in experimental microdiets on growth and survival of Japanese flounder larvae

A feeding experiment was conducted to investigate the effects of different ratios of EPA/AA in experimental microdiets on the growth,survival,body composition and tolerance to different stresses of Japanese flounder(Paralichthys olivaceus) larvae.Four kinds of experimental microdiets at four ratios of EPA/AA(5/1,2/1,1/1 and 0.4/1),with the same contents of n-3HUFA,DHA and EPA,were prepared. The results of 26-day feeding experiment indicated that the growth and survival of Japanese flounder larvae were optimal when the ratio of EPA/AA in experimental microdiet was 2/1.After the experiment,we analyzed fatty acids in bodies of larvae.The results showed that the content of AA in bodies of larvae increased with that in microdiets.