卡德藻自养、异养与兼养培养的比较研究

本文对卡德藻在自养、异养和兼养培养条件下的比生长速率、细胞密度、细胞色素组成及脂肪酸组成等方面进行了比较研究。兼养卡德藻的细胞密度大于自养条件和异养条件下细胞密度之和。兼养培养的卡德藻比生长速率是自养的 2倍 ,异养的 1.3倍。兼养和异养生长的对数期较自养的长。光合自养培养卡德藻最适光强为 10 0 0 0 lx,而兼养培养最适光强范围为 10 0 0～ 2 0 0 0 lx。在自养和兼养培养时亚油酸的含量很高 ,自养和兼养状态下分别为 16 .6 %和 17.4 9%。 EPA合成的量较低。异养藻的多不饱和脂肪酸的产量较自养藻和兼养藻要低。兼养藻细胞光合色素组成和含量与自养藻细胞的基本一致 ,但在异养条件下藻细胞色素组成发生明显改变。

Difference Between Autotrophic, Heterotrophic and Mixotrophic Growth of Tetraselmis sp

The growth rate, cell density, pigment composition and polyunsaturated fatty acid composition of Tetraselmis sp. were compared under autotrophic, heterotrophic and mixotrophic cultivation. The cell density of mixotrophic culture was higher than that of autotrophic and heterotrophic culture. The optimum light intensity under mixotrophic culture ranged from 1 000 lx to 2 000 lx, while that under autotrophic culture condition was about 10 000 lux. There were high contents of linoleic acids in auto and mixiotrophic culture, which were about 16.6% and 17.49% respectively, while there was a low content of EPA in them. The polyunsaturated fatty acids content of heterotrophic culture species was lower than that of auto and mixotrophic culture alga. The pigment composition under mixotrophic culture condition was nearly the same as that under autotrophic culture, but under heterotrophic culture condition the pigment composition changed dramatically.