| Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species, Zostera marina |
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| 作者姓名： | KONG Fann ZHOU Yang SUN Peipei LIU Limin MAO Yunxiang |
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| 作者单位： | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China |
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| 基金项目： | The Key Science and Technology Programof Shandong Province under contract No. 2012GHY11527; Natural Science Foundation of Shandong Province under contract No. Q2007E02; Specialized Research Fund for the Doctoral Program of Higher Education (New Teachers) under contract No. 20070423027; the Public Science and Technology Research Funds Projects ofOcean, StateOceanic Administration of the People's Republic of China under contract No. 201105021-8. |
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| 摘 要： | Zosteramarina, a monocotyledonous angiosperm, is one of themost important seagrass species. To investigate the salt-tolerancemechanismand discover salt-tolerant genes in Z. marina, a cDNA library was constructed. Single-pass sequencing of the 5' ends of 4 081 clones yielded 4 002 high quality expressed sequence tags (ESTs), which were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. The average length of the ESTs was 582 bp, with sizes ranging from100-1 500 bp. Basic Local Alignment Search Tool (BLASTX) analysis revealed that 1 664 unigenes had significant homology to known genes in the National Center for Biotechnology Information (NCBI) non-redundant (nr) database (E-value≤5-10). Among them, the twomost abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), accounting for 7.1% and 1.7% of the total ESTs, respectively. Using Kyoto Encyclopedia of Genes and Genomes (KEGG), 1 462 unigeneswere assigned to 1 161 pathways (E-value≤5-10). A total of 938 unigenes were assigned Gene Ontology (GO) terms based on the GO hierarchy analysis, and InterProScan searches recognized 1 003 InterPro families. Three genes formetallothionein in Z. marina that belonged to Class II was identified. Results of this study will improve understanding of the molecular mechanisms of saline tolerance in Z.marina.
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| 关 键 词： | 表达序列标签 耐盐基因 金属硫蛋白基因 EST序列 物种 苦草 游艇码头 藻 |
| 收稿时间： | 2012/3/22 0:00:00 |
| 修稿时间： | 2013/2/28 0:00:00 |
Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species, Zostera marina |
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| KONG Fann,ZHOU Yang,SUN Peipei,LIU Limin,MAO Yunxiang.Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species, Zostera marina[J].Acta Oceanologica Sinica,2013,32(8):68-78. |
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| Authors: | KONG Fann ZHOU Yang SUN Peipei LIU Limin and MAO Yunxiang |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Zosteramarina, a monocotyledonous angiosperm, is one of themost important seagrass species. To investigate the salt-tolerancemechanismand discover salt-tolerant genes in Z. marina, a cDNA library was constructed. Single-pass sequencing of the 5' ends of 4 081 clones yielded 4 002 high quality expressed sequence tags (ESTs), which were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. The average length of the ESTs was 582 bp, with sizes ranging from100-1 500 bp. Basic Local Alignment Search Tool (BLASTX) analysis revealed that 1 664 unigenes had significant homology to known genes in the National Center for Biotechnology Information (NCBI) non-redundant (nr) database (E-value≤5-10). Among them, the twomost abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), accounting for 7.1% and 1.7% of the total ESTs, respectively. Using Kyoto Encyclopedia of Genes and Genomes (KEGG), 1 462 unigeneswere assigned to 1 161 pathways (E-value≤5-10). A total of 938 unigenes were assigned Gene Ontology (GO) terms based on the GO hierarchy analysis, and InterProScan searches recognized 1 003 InterPro families. Three genes formetallothionein in Z. marina that belonged to Class II was identified. Results of this study will improve understanding of the molecular mechanisms of saline tolerance in Z.marina. |
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| Keywords: | expressed sequence tags (ESTs) gene function metallothionein salt-tolerant genes Zostera marina |
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