不同类型Zn胁迫对1株好氧反硝化细菌功能影响的比较研究

本文通过分析在不同浓度普通氧化锌(ZnO)、硫酸锌(ZnSO4)及纳米氧化锌(ZnO-NPs)胁迫下1株筛自胶州湾的好氧反硝化菌Zobellella sp.B307生长量和反硝化能力的变化,比较研究了不同类型Zn对该菌株生长及脱氮功能的影响。结果表明,ZnO-NPs对该菌株的生长有明显的持续抑制作用,显著降低其最大生长量,并对其脱氮功能产生持续抑制;ZnSO₄对菌株生长及脱氮功能前期抑制作用较强,后期抑制效应逐渐减弱;普通ZnO对菌株生长及脱氮功能并不产生显著影响。3种类型锌对该菌株的功能抑制表现为ZnO-NPs抑制作用最强,其次是ZnSO₄,普通ZnO的抑制相对较弱,产生影响差异的主要原因与这三种类型Zn化合物作用机制的不同有关。研究结果可为深入探讨不同类型的Zn污染对近海沉积物氮素循环产生的潜在影响提供依据。

Toxicity Effects of Different Types of Zn Stress on a Strain of Aerobic Denitrifying Bacteria Compare Research

To study the effects of different types of Zn on the growth and denitrification of an aerobic denitrifying bacterium Zobellella sp.B307 isolated from Jiaozhou Bay,the changes of strain growth and denitrification capacity under different concentrations of common zinc oxide(ZnO),zinc sulfate(ZnSO₄)and nano-zinc oxide(ZnO-NPs)were compared in this article.The results showed that ZnO-NPs had a significant sustained inhibitory effect on the growth of this strain,significantly reduced its maximum growth amount,and sustainedly inhibited its denitrification function.ZnSO₄ inhibited the growth and denitrification of the strain in the early stage,while the inhibitory effect was gradually weakened in the later stage.Ordinary ZnO has no significant effect on the strain.The functional inhibition of the three types of zinc on this strain showed that ZnO-NPs had the strongest inhibitory effect,followed by ZnSO4,and ordinary ZnO showed relatively weak inhibition,which is mainly related to the different mechanisms of action of these three types of Zn.The results of the study can provide a basis for further exploring the potential impact of different types of Zn pollution on the nitrogen cycle of offshore sediment.