沙蜇(Stomopholus meleagris)刺胞毒素对戒断小鼠超氧化物歧化酶和过氧化氢酶活性的作用

通过腹腔注射沙蜇刺胞毒素于吗啡依赖型戒断小鼠,检测小鼠肝脏、肾脏、心脏和脑的SOD、CAT、NOS酶活力和MDA含量的变化情况,并进行统计学分析,研究沙蜇刺胞毒素对纳洛酮催促吗啡依赖小鼠的戒断作用。结果表明,沙蜇刺胞毒素对吗啡依赖小鼠各组织酶活力的恢复有明显的作用。出现戒断症状的小鼠肝脏的SOD和CAT活性比对照组明显降低,注射沙蜇刺胞毒素后两种酶活性逐渐恢复,但还是略低于对照组。相反MDA含量吗啡组最高,沙蜇刺胞毒素Ⅰ次之,沙蜇刺胞毒素Ⅱ最低。通过小鼠戒断前后肝脏、肾脏、心脏、脑等组织中SOD和CAT活性酶的检测结果,证明了沙蜇刺胞毒素可改善小鼠由于吗啡作用而产生的机体的抗性下降、逐渐恢复正常生理功能,能抑制吗啡依赖小鼠戒断反应的发生,对吗啡依赖小鼠的戒断起到积极的治疗作用。

EFFECT OF CNIDOPHORIN OF STOMOLOPHUS MELEAGRIS ON MORPHINE DEPENDENT MICE

For exploring the use of marine animal toxin for drug addiction treatment and developing new side-effect-free anti-drug medicines, the effect of cnidophorin extracted from Stomopholus meleagris was tested on morphine-dependent mice. In our study, 4 experimental groups were set: 1. Control Group injected with normal saline (NS); 2. Morphine Group (Mor) injected with morphine in a designed strategy for setting up a morphine-dependent model; 3. Cnidophorin Group I injected with cnidophorin once (ST1); and 4. Cnidophorin Group II injected with cnidophorin twice (ST2). For observing drug-withdrawal syndrome, the Mor mice were injected with naloxone (Nal) after morphine and saline treatment for NS and Mor mice, and then the contents of superoxide dismutase, nitric oxide synthetase, catalase and malondiadehyde were measured. Results show that for morphine-treated mice, the activities of superoxide dismutase and catalase in liver, kidney and heart were reduced, the activity of nitric oxide synthetase in brain was also decreased, and radical cleaning in antioxidation was weakened as well. However, the ST1 and ST2 mice regained the activity of the enzymes and the mice resumed normal function gradually after the cnidophorin treatment. Therefore S. meleagris cnidophrin can be utilized to cure morphine dependent mice, which may have medical potential for treading drug-addiction for human patient in future.