海南红树林根系土壤中可培养细菌的多样性分析

为研究海南红树林根系土壤细菌多样性,充分发掘中国红树林特有微生物资源,作者于2012年12月份从10份采集于海南北港岛红树林根系淤泥样品中分离可培养海洋细菌,并基于16S rRNA基因序列分析的方法对样品中的细菌多样性进行研究。共分离得到122株海洋细菌,经并菌后选取其中的70株代表性菌株测序,结果发现它们分为4个类群:变形菌门(主要为γ-变形菌纲)(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria),分别占66%,19%,11%和4%。共代表30个属47个种,其中有9个潜在新种和2个潜在新属。本研究结果表明,北港岛红树林中存在着丰富的细菌多样性,很多类群可能是红树林特有细菌物种。

Diversity of culturable bacteria from the soil of root system of mangrove forest of Beigang island in Hainan Province

To investigate the diversity of soil bacteria in the root system of mangrove forest and potential microbacteria sources in Hainan Province, we collected ten soil samples from Beigang island in December 2012. The samples were treated by the plate dilution method to isolate culturable marine bacteria. The diversity of marine bacteria was examined based on 16S rRNA gene sequence analysis. Totally 70 representative strains were selected from 122 isolates, and they were further sequenced and subjected to phylogenetic analysis. The bacterial population was composed of 4 major lineages, Proteobacteria (66%, with a predominance of Gammaproteobacteria), Bacteroidetes (19%), Firmicutes (11%) and Actinobacteria (4%). These culturable bacterial isolates belonged to 30 genera and 47 species. There were 9 strains identified as potential novel species and 2 strains were identified as potential novel genus. This study indicated that there was high bacterial diversity in the soil of root system of mangrove forest of Beigang island and many isolates were the characteristic groups in mangrove forest.