| 褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究 |
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| 引用本文： | 徐晖,肖志忠,孔晓瑜,李军,董晓丽.褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究[J].热带海洋学报,2007,26(5):60-63. |
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| 作者姓名： | 徐晖 肖志忠 孔晓瑜 李军 董晓丽 |
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| 作者单位： | 1. 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003
2. 中国科学院海洋研究所,山东,青岛,266071 |
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| 摘 要： | 以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。
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| 关 键 词： | 褐牙鲆Paralichthys olivaceus 夏鲆P.dentatus 杂交 母系遗传 线粒体DNA |
| 文章编号： | 1009-5470（2007）05-0060-04 |
| 收稿时间： | 2006-12-15 |
| 修稿时间： | 2007-01-30 |
Analyses of mitochondrial 16S rDNA sequence in Paralichthys olivaceus(♀), P. dentatus(♂)and their hybrids |
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| XU Hui,XIAO Zhi-zhong,KONG Xiao-yu,LI Jun,DONG Xiao-li.Analyses of mitochondrial 16S rDNA sequence in Paralichthys olivaceus(♀), P. dentatus(♂)and their hybrids[J].Journal of Tropical Oceanography,2007,26(5):60-63. |
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| Authors: | XU Hui XIAO Zhi-zhong KONG Xiao-yu LI Jun DONG Xiao-li |
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| Institution: | Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China; 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | Partial 16S rRNA gene sequences of mitochondrial DNA were obtained from 5 females of Paralichthys olivaceus,5 males of P.dentatus and 11 individuals of their hybrids using PCR.The aligned length consisted of 590 base pairs.The variable sites were 28 and all the sites were parsimony informative.The sequence was identical in all the specimens of hybrids and the 3 analyzed P.olivaceus samples.Male-transmitted mitochondrial DNA was not detected in hybrid flounders.The results indicate that mitochondrial DNA is inherited maternally in hybridization between P.olivaceus and P.dentatus. |
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| Keywords: | Paralichthys olivaceus P dentatus hybridization mitochondrial DNA |
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