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烃降解菌假交替单胞菌N289的全基因组序列及其进化关系
引用本文:刘芳明,王以斌,曲长风,郑洲,缪锦来,许华,肖天.烃降解菌假交替单胞菌N289的全基因组序列及其进化关系[J].海洋学报(英文版),2017,36(2):88-93.
作者姓名:刘芳明  王以斌  曲长风  郑洲  缪锦来  许华  肖天
作者单位:中国科学院海洋研究所, 青岛, 266071, 中国;中国科学院大学, 北京, 100049, 中国;国家海洋局第一海洋研究所, 266061, 中国,国家海洋局第一海洋研究所, 266061, 中国,国家海洋局第一海洋研究所, 266061, 中国,国家海洋局第一海洋研究所, 266061, 中国,国家海洋局第一海洋研究所, 266061, 中国,中国水产科学研究院黄海水产研究所农业部渔业可持续发展重点实验室, 青岛, 266071, 中国,中国科学院海洋研究所, 青岛, 266071, 中国
基金项目:The National Natural Science Foundation of China under contract Nos 31200097, 41576187, U1406402-5 and 31202024; the Basic Scientific Fund for National Public Research Institutes of China under contract Nos 2013G33 and 2015G10.
摘    要:假交替单胞菌属归属于交替假单胞菌科。交替假单胞菌N289菌株分离自南极海冰,我们通过二代测序技术获得了其全基因组序列,组装后获得2条染色体和1条质粒,大小分别是3.2M、636kb和1.8kb。全基因组共包含3,589个ORF,GC含量为40.83%。基因功能分析表明,该菌酶活性高,具有很强的环境适应性。这项研究有助于了解生物多样性、该菌株的进化地位和微生物相互作用关系。

关 键 词:假交替单胞菌  低温  环境适应性  测序  进化关系
收稿时间:2015/11/1 0:00:00
修稿时间:2016/2/18 0:00:00

The complete genome of hydrocarbon-degrading Pseudoal-teromonas sp. NJ289 and its phylogenetic relationship
LIU Fangming,WANG Yibin,QU Changfeng,ZHENG Zhou,MIAO Jinlai,XU Hua and XIAO Tian.The complete genome of hydrocarbon-degrading Pseudoal-teromonas sp. NJ289 and its phylogenetic relationship[J].Acta Oceanologica Sinica,2017,36(2):88-93.
Authors:LIU Fangming  WANG Yibin  QU Changfeng  ZHENG Zhou  MIAO Jinlai  XU Hua and XIAO Tian
Institution:Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China;The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China and Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:Genus Pseudoalteromonas belongs to Family Pseudoalteromonadaceae in Gammaproteobacteria. A cold-adapted gram-negative bacterium, hydrocarbon-degrading Pseudoalteromonas sp. NJ289, was isolated from sea-ice of the Antarctica region, and sequenced the whole genome through the next generation sequencing platform. The assembly yielded three contigs representing two chromosomes and one plasmid with the sizes of 3.2 Mb, 636 kb and 1.8 kb, respectively. The G+C contents of genome were 40.83% and included 3 589 ORFs. Functional annotation indicated some potential roles in enzymatic activity and environmental adaptability. This study may help for understanding the population diverse, evolutionary ecology and the microbial interaction.
Keywords:Pseudoalteromonas  low temperature  environmental adaptability  sequencing  phylogenetic tree
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