氯吡苯脲对红砂愈伤组织诱导和增殖的影响及响应面法优化

以红砂的一年生苗嫩茎为外植体,添加不同浓度的氯吡苯脲（CPPU）、6-BA和NAA，进行愈伤组织诱导试验，得出红砂愈伤组织诱导的最佳培养基为1/2MS+CPPU 0.1 mg·L-1+NAA1 mg·L-1。利用响应面分析法，设计CCD中心组合试验，对CPPU和NAA两个因素进行优化，得出愈伤组织最佳继代培养基为1/2MS+CPPU 0.1195 mg·L-1+NAA1.1 mg·L-1，在此条件下，实际增殖率可达到4.7414，为理论预测值的99.15%，愈伤组织的最适继代周期为30 d左右。利用响应面分析法可优化红砂愈伤组织继代培养基，得到理论最佳配方，验证结果表明该方法科学、高效。

Effects of Forchlorfenuron on Callus Induction and Proliferation of Reaumuria soongorica and Its Formula Optimization by Response Surface Method

One-year old stem of Reaumuria soongorica was used as the explant to study callus induction and proliferation of desert plant Reaumuria soongorica by different CPPU,6-BA and NAA concentrations.Results indicate that callus is well induced on 1/2 MS agar medium supplemented with 0.1 mg·L-1 CPPU and 1 mg·L-1 NAA.According to the principles of Response Surface Method,2 factors and 5 levels are adopted in central composite design experiment.The experiment shows that optimal condition for callus proliferation is 1/2 MS agar medium with 0.1195 mg·L-1 CPPU and 1.1 mg·L-1 NAA.Under this condition,the callus proliferation rate is up to 4.7414 and it is 99.15% of the predictive value.The optimal period of the secondary culture of the calluses is 30 days.The research suggests that callus proliferation medium can be optimized by the Response Surface Method,and the proliferation rate can be increased,so the new method is effective and economical.