病原性副溶血弧菌在天然海水中的饥饿耐受研究

将副溶血弧菌培养至对数生长期,用天然海水洗脱,调整菌浓度至107个/cm3左右后置于27℃恒温培养箱进行饥饿试验.结果表明:饥饿初期细菌总数、可培养菌数和活菌数都有较大幅度上升;饥饿中、后期,总菌数与活菌数缓慢下降,而可培养菌数下降速度较快.饥饿前副溶血弧菌呈短杆状,染色均匀;饥饿后许多细胞中央出现染色较浅的区域,细胞形态变为椭圆形.副溶血弧菌对大黄鱼表皮黏液的黏附量随着饥饿时间延长而急剧下降,饥饿7 d后的黏附量接近于空白.用SDS-PAGE分析不同饥饿时期菌体蛋白质的差异,结果表明:饥饿30 d后菌体蛋白条带比饥饿前的蛋白质条带少;饥饿60 d后菌体蛋白质条带比饥饿30 d时的多,但比饥饿前少.研究结果对于揭示副溶血弧菌的流行病学特征有重要的参考价值.

Starvation responses of pathogenic Vibrio parahaemolyticus in natural Seawater

Pathogenic V.parahaemolyticus cells in log-phase were collected and the concentration was adjusted to 107 cells/cm3 in natural sea water for starvation studies.The results showed that: total bacteria number、viable bacteria number and CFU number of V.parahaemolyticus increased remarkably at the initial starvation stages;total bacteria number、viable bacteria number and CFU number fell at metaphase and anaphase,among them,the total bacteria number and viable bacteria number fell slowly while the CFU number fell more quickly.Before starvation,the V.parahaemolyticus cells were shortrods and dyed equably;after starvation,a light area appeared at the cell centre and some cells turned into oval.The amount of V.parahaemolyticus cells adhered to the large yellow croaker epidermal mucus dropped sharply during starvation,approaching the level of the control after 7 days starvation.The cellular protein of V.parahaemolyticus was extracted and analyzed by SDS-PAGE,the results showed that: bacteria starved for 30 d had less protein bands in the gel than the unstarved ones;bacteria starved for 60 d had more protein bands compared to those starved for 30 d,but less than the unstarved ones.The results obtained were useful to the underst anding of the epidemiologic characteristics of V.parahaemolyticus.