盐胁迫对盐芥悬浮培养细胞超微结构的影响

 以盐芥悬浮细胞为实验材料建立“悬浮细胞/盐胁迫”体系,并运用透射电镜技术对盐胁迫下盐芥悬浮细胞超微结构的变化进行了观察。结果表明,未胁迫下,盐芥细胞中各细胞器结构完整; 300 mM NaCl胁迫6 h后,开始出现质壁分离,细胞质具有较高的电子密度; 胁迫12 h后,出现明显的质壁分离现象,线粒体数目增多,多数变成月牙形 ,内部模糊不清,高尔基体失去正常形态,变得松散,“反式”面分泌小泡以“出芽”方式出现,同时发现胞内有“吞噬泡”存在。实验说明盐芥悬浮细胞能短时间耐受高浓度(300 mM)的氯化钠胁迫,当胁迫时间延长为12 h,细胞受损严重,亚细胞水平表现在细胞超微结构的改变,细胞“自我吞噬”可能在盐芥细胞盐胁迫响应过程中发挥重要作用。

Effect of Salt Stress on Ultrastructure of Thellungiella halophila Suspension-cultured Cells

Thellungiella halophila suspension-cultured cells were used to establish a 'cell culture/salt stress' system,and electronic transmission microscope was used to examine ultrastructural changes of Thellungiella halophila cells.Organelles remained normal structures in untreated cells,while the plasma membrane began to detach from the cell wall and the cytoplasm appeared electron-opaque when cells were stressed under 300 mM NaCl for 6 hours.The plasma membrane retracted apparently from the cell wall after salt ...