Assessing the germplasm of Laminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method |
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Authors: | He Yingjun Zou Yuping Wang Xiaodong Zheng Zhiguo Zhang Daming and Duan Delin |
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Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China |
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Abstract: | Eighteen gametophytes includingL. japonica, L. ochotensis andL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers
selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance
among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic
(UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively.
It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria, but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship
if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship
among the species ofLaminaria.
Contribution No. 4389 from the Institute of Oceanology, Chinese Academy of Sciences.
This research was supported by the State's High-tech Project of China, Marine 863 project (No. 819-03-02) and (2001AA621090). |
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Keywords: | Laminaria gametophyte RAPD germplasm identification |
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