动物蛋白酶解物与无机氮、磷源的组合对小球藻(Chlorella vulgaris)生长和生化指标的影响

采用三因素不完全正交设计方法,通过对细胞密度、干重、叶绿素和可溶性蛋白质含量的测定.确定了N、P源与蛋白酶解物之间的最佳组合,研究碳酸氢铵作为N源、磷酸二氢钠作为P源与动物蛋白酶解物之间的组合对小球藻生长和生化指标的影响.结果表明:(1)动物蛋白酶解物与N、P源间存在互作效应--影响小球藻细胞密度、叶绿素的主要因素分别是P浓度、蛋白酶解物浓度;而N浓度及蛋白酶解物浓度同时对小球藻的蛋白质含量起主要作用;(2)同时添加N 50mg/L、P 4mg/L、蛋白酶解物12ml/L能显著提高小球藻生物量,促进叶绿素和藻体蛋白质的合成(P<0.05).

ANIMAL PROTEIN ENZYMATIC HYDROLYSIS, N, AND P SOURCES: COMBINATION EFFECT ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHLORELLA VULGARIS

The effects of different combinations of animal protein enzymatic hydrolysis and N (NH₄HCO₃), P (NaH₂PO₄) on growth and biochemical parameters of Chlorella vulgaris are studied. The optimal combinations were determined by the index of biomass, chlorophyll content and protein content by orthogonal design test. The results show that: (1) Interaction occurred among protein enzymatic hydrolysis, N, and P sources. The influencing factors on the biomass were P concentration and protein enzymatic hydrolysis, and those on the protein content of C. vulgaris was the combination of protein enzymatic hydrolysis and N concentration. (2) The optimal combination conditions (P<0.05) to increase the biomass, chlorophyll content and protein content of C. vulgaris are: N concentration at 50mg/L, P at 4mg/L, and protein enzymatic hydrolysis at 12ml/L.