半连续培养氮磷浓度、更新率对三角褐指藻采收量的影响

采用均匀设计，在室温下研究了半连续培养中氮、磷浓度，更新率对三角褐指藻采收量的动态变化关系。结果显示，对１６ｄ更新期，氮、磷浓度分别在０．５～３５．９ｍｇ／Ｌ和０．３～１２．１ｍｇ／Ｌ范围对采收量无影响，但氮浓度在８ｄ前宜用０．５ｍｇ／Ｌ。更新率增加，藻浓度下降，藻生长率增长。据１６ｄ方程，最适更新率为１８．２％，每ｍｌ培养体积日采收量可达６６７０００细胞。

EFFECTS OF NITROGEN, PHOSPHORUS CONCENTRATION AND RENEWAL RATE ON RECOVERY YIELDS OF Phaeodactylum tricornutum IN SEMICONTINUOUS CULTURES

This experiment was conducted to determine the dynamic effects of nitrogen, phosphorus concentration and renewal rate on recovery yields of Phaeodactylum tricornutum in semicontinuous cultures at room temperature by uniform design. The results demonstrated that nitrogen concentration ranging from 0.5 rg / L to 35.9 rg/L and plosphorus concentration ranging from 0.3 mg/L to 12.1 mg/L had no influences upon recovery yields during 16 d dilution, but 0.5 rg/L of nitrogen concentration was suggested before 8 d. Cell density decreased and growth rate increased while increasing renewal rate. The optimal renewal rate of 18.2% was postulated according to 16 d equation, and recovery yields of 66.7×104 cells per ml volume of culture per day were obtained with this renewal rate.