Recovery of astaxanthin from discharged wastewater during the production of chitin |
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| Authors: | Xiaolin Chen Shengfeng Yang Ronge Xing Huahua Yu Song Liu Pengcheng Li |
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| Institution: | CHEN Xiaolin1), 2), YANG Shengfeng2), XING Ronge3), YU Huahua3), LIU Song 3), and LI Pengcheng 3),* 1) Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, P. R. China 2) Qingdao Tumor Hospital, Qingdao 266000, P. R. China 3) Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P. R. China |
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| Abstract: | In this paper, studies were carried out to extract astaxanthin from discharged wastewater during the production of chitin and to reveal the scavenging effect of the obtained pigment on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. Different ratios of dichloromethane/methanol (V/V) were used to extract astaxanthin. When the ratio of dichloromethane/methanol was 2:8 and the ratio between the mixed organic solvent (dichloromethane/methanol, 2:8, V/V) and wastewater was 1:1, the highest yield of pigment was obtained (8.4 mg/ 50 mL). The concentration of free astaxanthin in the obtained pigment analyzed by HPLC was 30.02%. The obtained pigment possessed strong scavenging ability on DPPH radical and IC50 was 0.84mg/ml. |
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| Keywords: | dichloromethane/methanol astaxanthin extract pigment wastewater |
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