Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from <Emphasis Type="Italic">Venerupis philippinarum</Emphasis> to heavy metals and benzo[a]pyrene exposure |
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Authors: | Linbao?Zhang Email author" target="_blank">Huifeng?WuEmail author Xiaoli?Liu Leilei?Chen Qing?Wang Email author" target="_blank">Jianmin?ZhaoEmail author Liping?You |
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Institution: | 1, 2 1 Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, China 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract: | Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play
important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega
GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids
with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded
to heavy metals and benzoa]pyrene (Ba]P) exposure were investigated by quantitative real-time RT-PCR. The expression of
VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants.
Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum
6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For Ba]P exposure, the maximum
induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement
of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and Ba]P exposure. |
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Keywords: | Venerupis philippinarum Glutathione S-transferase mRNA expression heavy metals benzo[a]pyrene biomarker |
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