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| 红树林沉积物来源链霉菌HA6菌株发酵条件的优化 | |
| 引用本文： | 肖静,洪专. 红树林沉积物来源链霉菌HA6菌株发酵条件的优化[J]. 台湾海峡, 2011, 30(4): 589-593. DOI: 10.3969/J.ISSN.1000-8160.2011.04.021 |
| 作者姓名： | 肖静 洪专 |
| 作者单位： | 国家海洋局第三海洋研究所,福建厦门,361005 |
| 基金项目： | 国家海洋局第三海洋研究所基本科研业务费专项资金资助项目 |
| 摘 要： | HA6菌株是分离自红树林沉积物中具有强抗菌活性的链霉菌．为提高其抗菌活性物质的产量，通过单因子试验及正交试验法对其包括发酵培养基的组成、接种量、通气量和发酵时间等发酵条件进行优化．研究表明其最佳摇瓶发酵培养基配方为：麦芽提取物质量为2．00g、可溶性淀粉质量为3．00g、豆饼粉质量为2．00g、（NH4）：SO。质量为1．00g、NaCl质量为3．00g、CaC03质量为0．50g、蒸馏水体积为100cm^3；最优发酵条件为：500em。摇瓶装液体积为150em。，接种量为8％，培养温度为28℃，初始pH为7．5，转速为200r／rain，培养时间为5d． |
| 关 键 词： | 海洋生物学 红树林 链霉菌 抗茵活性物质 发酵 优化 |
Optimization of fermentation conditions of Streptomyces HA6 isolated from mangrove sediment |
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| XIAO Jing,HONG Zhuan. Optimization of fermentation conditions of Streptomyces HA6 isolated from mangrove sediment[J]. Journal of Oceanography In Taiwan Strait, 2011, 30(4): 589-593. DOI: 10.3969/J.ISSN.1000-8160.2011.04.021 | |
| Authors: | XIAO Jing HONG Zhuan |
| Affiliation: | XIAO Jing,HONG Zhuan(Third Institute of Oceanography,SOA,Xiamen 361005,China) |
| Abstract: | Strain HA6,which was isolated from mangrove sediments,showed strong antibacterial activity.16S rDNA gene analysis indicated that strain HA6 belongs to genus Streptomyces and shared highest 98.8% similarities with Streptomyces griseorubens.To improve its production of antibacterial substance,single factor experiment and orthogonal experiment were used to optimize the fermentation conditions considering the medium ingredients,inoculation concentration,ventilatory capacity and fermentation time.The results ind... |
| Keywords: | marine biology mangrove Streptomyces antibacterial substances fermentation optimization |
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