基于离散三维活体荧光光谱法的湖泊水体叶绿素a浓度原位测量

荧光光谱分析技术具有灵敏度高、检测快速等优点,三维荧光光谱提供的指纹荧光信息比普通荧光光谱更丰富,选择性更好,在多组分分离上更具优势.离散三维活体荧光光谱法通过提取水体蓝藻、绿藻、硅藻、甲藻和隐藻5个门类藻类荧光光谱的指纹特征,分类测量藻类叶绿素a浓度,经过加和得到总的水体叶绿素a浓度.将基于该方法研制的三维荧光光谱水体藻类原位测量仪用于太湖水体叶绿素a浓度的测定,并与YSI多参数水质检测仪、BBE藻类现场分析仪、分光光度法等测定结果对比,结果表明:该方法与分光光度法间无显著性差异,与分光光度法、BBE法间的相关性好,相关系数达0.96以上,精密度、准确率优于基于普通荧光法的原位测量仪,是一种快速有效的原位测量方法.

In-situ measurement of chlorophyll-a concentration in the lake based on discrete three-dimensional fluorescent spectroscopy in vivo

Fluorescence spectroscopy had the advantages of high sensitivity and fast detecting. The three-dimensional(3D)fluorescence spectrometry was more informative, better selectivity, more advantage than the general fluorescence spectroscopy on the multicomponent separation. The determination method of chlorophyll-a in water based on the discrete three-dimensional fluorescence spectrometry in vivo was studied. It extracted different fluorescence fingerprint properties of five divisions of algae (Cyanophyta, Chlorophyta, Bacillariophyta, Dinophyta and Cryptophyta), measured chlorophyll-a concentration of every algae and got the total chlorophyll-a concentration by plus. The three-dimensional fluorescence algae in-situ analyzer (AFA) was manufactured based on it. The chlorophyll-a concentration in Lake Taihu was measured by AFA. The measured values were compared with YSI, BBE and the spectrophotometry. It showed no significant difference between AFA and the spectrophotometry. There were good correlation among AFA, spectrophotometry and BBE. The correlation coefficient was above of 0.96. AFA was better precision and accuracy than the general fluorescence spectroscopy. It was a fast and effective in-situ method.